Document Detail

Universal Linkage System: versatile nucleic acid labeling technique.
MedLine Citation:
PMID:  11901803     Owner:  NLM     Status:  MEDLINE    
Over the last two decades nonradioactive nucleic acid labeling and detection systems have overcome the safety, disposal, stability and cost problems that are associated with radioactive techniques. Besides traditional, enzyme-mediated, nonradioactive labeling methods (e.g., random priming, nick translation or labeling by PCR), several chemical labeling systems have been developed (e.g., ULS, psoralen, alkylating agents). These methods provide fluorescent (or hapten) labeled probes for fluorescence in situ hybridization (FISH), comparative genomic hybridization (CGH) and microarray-based techniques. In this review the DNA-based molecular diagnostic applications and perspectives of the Universal Linkage System (ULS) technology will be described.
R P van Gijlswijk; E G Talman; P J Janssen; S S Snoeijers; J Killian; H J Tanke; R J Heetebrij
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Review    
Journal Detail:
Title:  Expert review of molecular diagnostics     Volume:  1     ISSN:  1473-7159     ISO Abbreviation:  Expert Rev. Mol. Diagn.     Publication Date:  2001 May 
Date Detail:
Created Date:  2002-03-20     Completed Date:  2002-04-26     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  101120777     Medline TA:  Expert Rev Mol Diagn     Country:  England    
Other Details:
Languages:  eng     Pagination:  81-91     Citation Subset:  IM    
Kreatech Diagnostics BV, PO Box 37078, 1030 AB Amsterdam, The Netherlands.
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MeSH Terms
Chlamydia trachomatis / genetics,  isolation & purification
DNA Probes
In Situ Hybridization, Fluorescence
Linkage (Genetics)
Molecular Diagnostic Techniques*
Nucleic Acids / chemistry*
Oligonucleotide Array Sequence Analysis
Papillomaviridae / genetics,  isolation & purification
RNA, Messenger
Staining and Labeling / methods*
Staphylococcus aureus / genetics,  isolation & purification
Reg. No./Substance:
0/DNA Probes; 0/Nucleic Acids; 0/RNA, Messenger

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