| A Universal HPLC-MS Method to Determine the Stereochemistry of Common and Unusual Amino Acids. | |
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MedLine Citation:
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PMID: 22125137 Owner: NLM Status: In-Data-Review |
Abstract/OtherAbstract:
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The determination of the stereochemistry of common and unusual amino acids is important in food chemistry, archeology, medicine, and life sciences, including such diverse areas as marine biology and extraterrestrial chemistry and has greatly contributed to our current knowledge in these fields.To determine the stereochemistry of amino acids, many chromatographic methods have been developed and refined over the last decades. Here, we describe a state-of-the-art indirect chromatography-based LC-MS method. Diastereomers were formed from amino acids that were reacted with chiral derivatizing agents, such as Marfey's reagent (FDAA), GITC, S-NIFE, and OPA-IBLC and separated on a reversed phase column using mass spectrometry compatible buffers. |
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Authors:
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Sonja Hess |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Methods in molecular biology (Clifton, N.J.) Volume: 828 ISSN: 1940-6029 ISO Abbreviation: Methods Mol. Biol. Publication Date: 2012 |
Date Detail:
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Created Date: 2011-11-29 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9214969 Medline TA: Methods Mol Biol Country: United States |
Other Details:
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Languages: eng Pagination: 63-75 Citation Subset: IM |
Affiliation:
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Proteome Exploration Laboratory, California Institute of Technology, BI 211, MC139-74, Pasadena, CA, USA, shess@caltech.edu. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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