Document Detail

Universal fractionation of lignin-carbohydrate complexes (LCCs) from lignocellulosic biomass: an example using spruce wood.
MedLine Citation:
PMID:  23332001     Owner:  NLM     Status:  MEDLINE    
It is of both theoretical and practical importance to develop a universally applicable approach for the fractionation and sensitive lignin characterization of lignin-carbohydrate complexes (LCCs) from all types of lignocellulosic biomass, both natively and after various types of processing. In the present study, a previously reported fractionation approach that is applicable for eucalyptus (hardwood) and flax (non-wood) was further improved by introducing an additional step of barium hydroxide precipitation to isolate the mannan-enriched LCC (glucomannan-lignin, GML), in order to suit softwood species as well. Spruce wood was used as the softwood sample. As indicated by the recovery yield and composition analysis, all of the lignin was recovered in three LCC fractions: a glucan-enriched fraction (glucan-lignin, GL), a mannan-enriched fraction (GML) and a xylan-enriched fraction (xylan-lignin, XL). All of the LCCs had high molecular masses and were insoluble or barely soluble in a dioxane/water solution. Carbohydrate and lignin signals were observed in (1) H NMR, (13) C CP-MAS NMR and normal- or high-sensitivity 2D HSQC NMR analyses. The carbohydrate and lignin constituents in each LCC fraction are therefore believed to be chemically bonded rather than physically mixed with one another. The three LCC fractions were found to be distinctly different from each other in terms of their lignin structures, as revealed by highly sensitive analyses by thioacidolysis-GC, thioacidolysis-SEC and pyrolysis-GC.
Xueyu Du; Goran Gellerstedt; Jiebing Li
Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.     Date:  2013-03-04
Journal Detail:
Title:  The Plant journal : for cell and molecular biology     Volume:  74     ISSN:  1365-313X     ISO Abbreviation:  Plant J.     Publication Date:  2013 Apr 
Date Detail:
Created Date:  2013-04-05     Completed Date:  2013-09-23     Revised Date:  2014-09-22    
Medline Journal Info:
Nlm Unique ID:  9207397     Medline TA:  Plant J     Country:  England    
Other Details:
Languages:  eng     Pagination:  328-38     Citation Subset:  IM    
Copyright Information:
© 2013 The Authors The Plant Journal © 2013 Blackwell Publishing Ltd.
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MeSH Terms
Carbohydrates / chemistry*
Lignin / chemistry*
Magnetic Resonance Spectroscopy
Picea / chemistry*
Grant Support
P41 GM066326/GM/NIGMS NIH HHS; P41 RR002301/RR/NCRR NIH HHS; P41GM66326/GM/NIGMS NIH HHS; P41RR02301/RR/NCRR NIH HHS; RR02781/RR/NCRR NIH HHS; RR08438/RR/NCRR NIH HHS; S10 RR002781/RR/NCRR NIH HHS; S10 RR002781-01/RR/NCRR NIH HHS; S10 RR008438/RR/NCRR NIH HHS; S10 RR008438-01/RR/NCRR NIH HHS; //Biotechnology and Biological Sciences Research Council
Reg. No./Substance:
0/Carbohydrates; 9005-53-2/Lignin

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