| Universal Chemiluminescence Flow-Through Device Based on Directed Self-Assembly of Solid-State Organic Chromophores on Layered Double Hydroxide Matrix. | |
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MedLine Citation:
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PMID: 23330845 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
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In this work, a universal chemiluminescence (CL) flow-through device suitable for various CL resonance energy transfer (CRET) systems has been successfully fabricated. Highly efficient CRET in solid-state photoactive organic molecules can be achieved by assembling them on the surface of layered double hydroxides (LDHs). We attribute these observations to the suppression of the intermolecular π-π stacking interactions among aromatic rings and the improvement of molecular orientation and planarity in the LDH matrix, enabling a remarkable increase in fluorescence lifetime and quantum yield of organic molecules. Under optimal conditions, using peroxynitrous acid-fluorescein dianion (FLUD) as model CRET system, trace FLUD (10 μM) were assembled on the surface of LDHs. Peroxynitrous acid/nitrite could be assayed in the range of 1.0 to 500 μM, and the detection limit for peroxynitrous acid/nitrite (S/N=3) was 0.6 μM. This CL flow-through device exhibited operational stability, high reproducibility and long lifetime. While LDHs were immobilized in a flow-through device in the absence of FLUD, the detection limit for peroxynitrous acid/nitrite was 100 μM. On the other hand, FLUD at the same concentration can not enhance the CL intensity of peroxynitrous acid system. This fabricated CL flow-through column has been successfully applied to determine nitrite in sausage samples with recoveries of 98-102%. These satisfactory results demonstrated that our studies pave a novel way towards flow-through column-based CRET using solid-state organic molecules as acceptors for signal amplification. |
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Authors:
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Zhihua Wang; Xu Teng; Chao Lu |
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Publication Detail:
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Type: JOURNAL ARTICLE Date: 2013-1-18 |
Journal Detail:
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Title: Analytical chemistry Volume: - ISSN: 1520-6882 ISO Abbreviation: Anal. Chem. Publication Date: 2013 Jan |
Date Detail:
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Created Date: 2013-1-21 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 0370536 Medline TA: Anal Chem Country: - |
Other Details:
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Languages: ENG Pagination: - Citation Subset: - |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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