Document Detail

Universal Chemiluminescence Flow-Through Device Based on Directed Self-Assembly of Solid-State Organic Chromophores on Layered Double Hydroxide Matrix.
MedLine Citation:
PMID:  23330845     Owner:  NLM     Status:  Publisher    
In this work, a universal chemiluminescence (CL) flow-through device suitable for various CL resonance energy transfer (CRET) systems has been successfully fabricated. Highly efficient CRET in solid-state photoactive organic molecules can be achieved by assembling them on the surface of layered double hydroxides (LDHs). We attribute these observations to the suppression of the intermolecular π-π stacking interactions among aromatic rings and the improvement of molecular orientation and planarity in the LDH matrix, enabling a remarkable increase in fluorescence lifetime and quantum yield of organic molecules. Under optimal conditions, using peroxynitrous acid-fluorescein dianion (FLUD) as model CRET system, trace FLUD (10 μM) were assembled on the surface of LDHs. Peroxynitrous acid/nitrite could be assayed in the range of 1.0 to 500 μM, and the detection limit for peroxynitrous acid/nitrite (S/N=3) was 0.6 μM. This CL flow-through device exhibited operational stability, high reproducibility and long lifetime. While LDHs were immobilized in a flow-through device in the absence of FLUD, the detection limit for peroxynitrous acid/nitrite was 100 μM. On the other hand, FLUD at the same concentration can not enhance the CL intensity of peroxynitrous acid system. This fabricated CL flow-through column has been successfully applied to determine nitrite in sausage samples with recoveries of 98-102%. These satisfactory results demonstrated that our studies pave a novel way towards flow-through column-based CRET using solid-state organic molecules as acceptors for signal amplification.
Zhihua Wang; Xu Teng; Chao Lu
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2013-1-18
Journal Detail:
Title:  Analytical chemistry     Volume:  -     ISSN:  1520-6882     ISO Abbreviation:  Anal. Chem.     Publication Date:  2013 Jan 
Date Detail:
Created Date:  2013-1-21     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0370536     Medline TA:  Anal Chem     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
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