Document Detail

Unit length as the denominator for quantitation of cell proliferation in nasal epithelia.
MedLine Citation:
PMID:  2194274     Owner:  NLM     Status:  MEDLINE    
Cell proliferation data, generally based on a labeling index (LI), provide a valuable endpoint for assessment of toxic and potentially carcinogenic responses in laboratory animals. Measurement of the LI is time consuming because of the large number of cells that need to be counted to determine the denominator. In respiratory mucosa, the total cell count of the surface epithelia may be altered in response to treatment, either through cell loss or increases in cell number (e.g., hyperplasia). As an alternative to the more conventional LI, the present studies were carried out to assess the value of expressing cell proliferation in nasal epithelia as a unit length labeling index (ULLI), defined as labeled cells per mm of basement membrane. Rats were exposed by inhalation to formaldehyde or methyl bromide, and changes in cell proliferation were determined in the respiratory and olfactory epithelia, respectively, using both total cell count and basement membrane length as denominators. Total cell counts were clearly influenced by treatment, while basement membrane length was not. Both methods revealed similar treatment-induced effects on cell proliferation, and in fact were highly correlated (R greater than or equal to 0.92, p less than 0.001). It was concluded that the ULLI method provides an effective alternative to total cell counts and the LI method. This approach is not influenced by alterations in the total cell population, and has the benefit of being less labor intensive than LI determinations.
T M Monticello; K T Morgan; M E Hurtt
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Toxicologic pathology     Volume:  18     ISSN:  0192-6233     ISO Abbreviation:  Toxicol Pathol     Publication Date:  1990  
Date Detail:
Created Date:  1990-08-08     Completed Date:  1990-08-08     Revised Date:  2009-07-01    
Medline Journal Info:
Nlm Unique ID:  7905907     Medline TA:  Toxicol Pathol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  24-31     Citation Subset:  IM    
CIIT, Research Triangle Park, North Carolina 27709.
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MeSH Terms
Administration, Inhalation
Basement Membrane / ultrastructure
Cell Count / drug effects,  methods
Cell Division
Cell Survival / drug effects
Epithelial Cells
Epithelium / drug effects,  ultrastructure
Formaldehyde / administration & dosage,  toxicity
Histological Techniques
Hydrocarbons, Brominated / administration & dosage,  toxicity
Hyperplasia / pathology
Nasal Cavity / cytology*,  drug effects,  ultrastructure
Rats, Inbred F344
Reg. No./Substance:
0/Hydrocarbons, Brominated; 50-00-0/Formaldehyde; 74-83-9/methyl bromide

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