| Unique requirement for Rb/E2F3 in neuronal migration: evidence for cell cycle-independent functions. | |
| | |
MedLine Citation:
|
PMID: 17452454 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
The cell cycle regulatory retinoblastoma (Rb) protein is a key regulator of neural precursor proliferation; however, its role has been expanded to include a novel cell-autonomous role in mediating neuronal migration. We sought to determine the Rb-interacting factors that mediate both the cell cycle and migration defects. E2F1 and E2F3 are likely Rb-interacting candidates that we have shown to be deregulated in the absence of Rb. Using mice with compound null mutations of Rb and E2F1 or E2F3, we asked to what extent either E2F1 or E2F3 interacts with Rb in neurogenesis. Here, we report that E2F1 and E2F3 are both functionally relevant targets in neural precursor proliferation, cell cycle exit, and laminar patterning. Each also partially mediates the Rb requirement for neuronal survival. Neuronal migration, however, is specifically mediated through E2F3, beyond its role in cell cycle regulation. This study not only outlines overlapping and distinct functions for E2Fs in neurogenesis but also is the first to establish a physiologically relevant role for the Rb/E2F pathway beyond cell cycle regulation in vivo. |
| | |
Authors:
|
Kelly A McClellan; Vladimir A Ruzhynsky; David N Douda; Jacqueline L Vanderluit; Kerry L Ferguson; Danian Chen; Rod Bremner; David S Park; Gustavo Leone; Ruth S Slack |
Related Documents
:
|
11325934 - The ccrm dna methyltransferase of agrobacterium tumefaciens is essential, and its activ... 22302684 - Increased radioresistance via g12s k-ras by compensatory upregulation of mapk and pi3k ... 15201494 - Effects of hmgb-1 overexpression on cell-cycle progression in mcf-7 cells. 15491914 - Cell cycle and differentiation. 7759994 - Follicular dendritic cells inhibit apoptosis in human b lymphocytes by a rapid and irre... 22225634 - Cinobufacini induced mda-mb-231 cell apoptosis-associated cell cycle arrest and cytoske... |
Publication Detail:
|
Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2007-04-23 |
Journal Detail:
|
Title: Molecular and cellular biology Volume: 27 ISSN: 0270-7306 ISO Abbreviation: Mol. Cell. Biol. Publication Date: 2007 Jul |
Date Detail:
|
Created Date: 2007-06-18 Completed Date: 2007-08-09 Revised Date: 2009-11-18 |
Medline Journal Info:
|
Nlm Unique ID: 8109087 Medline TA: Mol Cell Biol Country: United States |
Other Details:
|
Languages: eng Pagination: 4825-43 Citation Subset: IM |
Affiliation:
|
Department of Cellular and Molecular Medicine, Ottawa Health Research Institute, University of Ottawa, Ottawa, ON, Canada. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Animals Cell Cycle* Cell Movement* Cell Proliferation Cell Survival E2F1 Transcription Factor / metabolism E2F3 Transcription Factor / metabolism* Female Gene Expression Regulation Interneurons / cytology Membrane Proteins / metabolism Mice Mice, Inbred C57BL Mice, Knockout Neurons / cytology*, metabolism Oligonucleotide Array Sequence Analysis Protein Binding Retinoblastoma Protein / metabolism* Stem Cells / cytology, metabolism Telencephalon / embryology, metabolism |
| Chemical | |
Reg. No./Substance:
|
0/E2F1 Transcription Factor; 0/E2F3 Transcription Factor; 0/Membrane Proteins; 0/Retinoblastoma Protein; 0/neogenin |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: A small conserved domain of Drosophila PERIOD is important for circadian phosphorylation, nuclear lo...
Next Document: Yeast Rpb9 plays an important role in ubiquitylation and degradation of Rpb1 in response to UV-induc...