Document Detail


Unique protein expression by the TBJ clonal derivative of C1300 murine neuroblastoma.
MedLine Citation:
PMID:  8749920     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
C1300 is a murine neuroblastoma that arose spontaneously in an A/JAX mouse, and from which a clone termed TBJ was subsequently derived. C1300 is a slowly growing and poorly metastasizing tumor, whereas TBJ shows early systemic metastasis as well as aggressive local growth. Compared with TBJ cells, C1300 cells are highly immunogenic and are sensitive to natural killer cells and cytotoxic lymphocytes. In vitro, TBJ cells were found to be more rounded and less adherent than C1300 cells. Because the underlying basis for the differences between C1300 and TBJ cells has not been fully elucidated, the authors used high-resolution two-dimensional gel electrophoresis (2-DE) to study comparative aspects of total protein expression by each cell line. Of the approximately 400 individual cellular proteins that could be resolved using this technique, two were found to be reproducibly and uniquely expressed by TBJ cells and not by C1300 cells. Both proteins were anionic (pl 5.0 to 5.2) as assessed by iso-electric focusing and had molecular weights of 76,000 and 82,000 as assessed by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE). Silver staining of SDS-polyacrylamide gels showed that the levels of 82,000-M(r) protein (p82) were higher than those of the 76,000-M(r) protein (p76). A purification protocol allowing for the isolation of p82 from TBJ cell extracts was developed, which comprised preparative two-dimensional gel electrophoresis followed by reverse-phase high-performance liquid chromatography. Full molecular identification of p82 and p76 eventually may provide new leads in the study of the metastatic or antigenic properties of neuroblastoma.
Authors:
R K Vegunta; D R Cooney; M T Downing; G E Besner; D R Brigstock
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of pediatric surgery     Volume:  30     ISSN:  0022-3468     ISO Abbreviation:  J. Pediatr. Surg.     Publication Date:  1995 Dec 
Date Detail:
Created Date:  1996-09-20     Completed Date:  1996-09-20     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0052631     Medline TA:  J Pediatr Surg     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  1668-72     Citation Subset:  IM    
Affiliation:
Department of Surgery, Ohio State University College of Medicine, Columbus 43205, USA.
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MeSH Terms
Descriptor/Qualifier:
Animals
Antigens, Neoplasm / genetics*,  isolation & purification
Cell Division / genetics
Cell Line
Chromatography, High Pressure Liquid
Clone Cells / chemistry*
Electrophoresis, Gel, Two-Dimensional
Electrophoresis, Polyacrylamide Gel
Gene Expression Regulation, Neoplastic / physiology
Male
Mice
Molecular Weight
Neoplasm Transplantation
Neuroblastoma / genetics*
Chemical
Reg. No./Substance:
0/Antigens, Neoplasm

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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