Document Detail

Unique disialosyl gangliosides from salmon kidney: characterization of V3alphaFuc, IV3betaGalNAc, II3(alphaNeuAc)2-Gg4Cer and its analogue with 4-O-acetyl-N-acetylneuraminic acid.
MedLine Citation:
PMID:  17006641     Owner:  NLM     Status:  MEDLINE    
Four unidentified acidic glycolipids (X3-X6) were isolated from the kidney of the Pacific salmon on an anion exchange column and by high performance liquid chromatography using a silica bead (Iatrobeads) column. Based on methylation analysis, chemical and enzymatic degradation, proton nuclear magnetic resonance spectroscopy and mass spectrometry, the glycon structure of X5 and X6 was identified as a unique disialosyl fucosyl-N-acetylgalactosaminyl ganglio-N-tetraose:Fucalpha3GalNAcbeta3Galbeta3GalNAcbeta4[NeuAcalpha8NeuAcalpha3] Galbeta4Glcbeta1Cer. NMR showed that X3 and X4 were analogues of X5 and X6 and contained O-acetyl groups on C4 of the outer N-acetylneuraminic acid, first disialosyl gangliosides containing 4-O-acetyl-N-acetylneuraminic acid. The ceramides of X3 and X5 contained predominantly C24: 1, and X4 and X6 contained saturated fatty acids (C14: 0, C16: 0 and C18: 0), whereas the long chain base was exclusively sphingenine. The concentrations of X3 and X4 were 0.13 and 0.16 nmol/g of kidney respectively and those of X5 and X6, were 0.07 nmol/g each.
Yukio Niimura; Ineo Ishizuka
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Glycoconjugate journal     Volume:  23     ISSN:  0282-0080     ISO Abbreviation:  Glycoconj. J.     Publication Date:  2006 Nov 
Date Detail:
Created Date:  2006-09-28     Completed Date:  2007-04-11     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8603310     Medline TA:  Glycoconj J     Country:  United States    
Other Details:
Languages:  eng     Pagination:  489-99     Citation Subset:  IM    
Research Center of Biomedical Analysis and Radioisotope, Teikyo, Tokyo, Japan.
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MeSH Terms
Carbohydrate Sequence
Chromatography, High Pressure Liquid
Chromatography, Ion Exchange
Gangliosides / chemistry*,  isolation & purification
Gas Chromatography-Mass Spectrometry
Kidney / chemistry*
Magnetic Resonance Spectroscopy
Molecular Sequence Data
Molecular Structure
Oncorhynchus keta / metabolism*
Spectrometry, Mass, Secondary Ion
Reg. No./Substance:
0/Gangliosides; 0/sialogangliosides

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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