| Unique biological properties of Mycobacterium tuberculosis L-form variants: impact for survival under stress. | |
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MedLine Citation:
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PMID: 22847267 Owner: NLM Status: In-Data-Review |
Abstract/OtherAbstract:
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Bacteria can, under certain conditions, enter into a cell-less state known as L-form conversion. This phenomenon is universal, but also recognized with difficultly by microbiologists. The current study addresses several aspects concerning the ability of tubercle bacilli to use L-form conversion as a unique adaptive strategy to survive and reproduce under unfavorable conditions. Nutrient starvation of M. tuberculosis in vitro followed by passages in Middlebrook 7H9 semisolid medium was used for stress induction and the selective isolation of mycobacterial L-form variants. Light and electron microscopy images evidence the peculiar characteristics of mycobacterial L-forms. For example, mycobacterial L-forms were observed to lose their acid-fastness and change their morphology. In addition, wide morphological variability, the presence of large and elementary bodies, coccoids and small granular forms, as well as the appearance of unusual modes of irregular cell division were observed. Unlike classical tubercle bacilli, L-form variants grew and developed typical "fried-egg" colonies faster. L-forms were verified as M. tuberculosis by spoligotyping. The results provide insights into the nature of L-form phenomena in M. tuberculosis and link them to the mechanisms allowing mycobacterial survival under stress. |
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Authors:
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Nadya Markova; Georgi Slavchev; Lilia Michailova |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: International microbiology : the official journal of the Spanish Society for Microbiology Volume: 15 ISSN: 1618-1905 ISO Abbreviation: Int. Microbiol. Publication Date: 2012 Jun |
Date Detail:
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Created Date: 2012-07-31 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9816585 Medline TA: Int Microbiol Country: Spain |
Other Details:
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Languages: eng Pagination: 61-8 Citation Subset: IM |
Affiliation:
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Institute of Microbiology, Bulgarian Academy of Sciences, Sofia, Bulgaria. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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