Document Detail


Unique action of a modified weakly acidic uncoupler without an acidic group, methylated SF 6847, as an inhibitor of oxidative phosphorylation with no uncoupling activity: possible identity of uncoupler binding protein.
MedLine Citation:
PMID:  2894856     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The potent weakly acidic uncoupler SF 6847 was modified by methylation of its phenolic OH group, and the effect of the resulting derivative, with no acid-dissociable group, on oxidative phosphorylation in rat liver mitochondria was examined. The methylated SF 6847 did not induce uncoupling at up to 40 microM, while SF 6847 uncoupled oxidative phosphorylation completely at about 20 nM, indicating that the acid-dissociable group is essential for uncoupling. The O-methylated SF 6847 at 20 microM did, however, inhibit state 3 respiration of mitochondria, although it did not inhibit electron-flow through the respiratory chain, ATPase activated by weakly acidic uncouplers or Pi-ATP exchange. At the same concentration, it also inhibited ATP synthesis in submitochondrial particles. These features are different from those of known inhibitors of oxidative phosphorylation. Thus, O-methylated SF 6847 is a unique inhibitor of oxidative phosphorylation. The possible identity of the uncoupler binding protein is discussed on the basis of these results.
Authors:
H Terada; Y Fukui; Y Shinohara; M Ju-ichi
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Publication Detail:
Type:  Comparative Study; Journal Article    
Journal Detail:
Title:  Biochimica et biophysica acta     Volume:  933     ISSN:  0006-3002     ISO Abbreviation:  Biochim. Biophys. Acta     Publication Date:  1988 Mar 
Date Detail:
Created Date:  1988-05-11     Completed Date:  1988-05-11     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0217513     Medline TA:  Biochim Biophys Acta     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  193-9     Citation Subset:  IM    
Affiliation:
Faculty of Pharmaceutical Sciences, University of Tokushima, Japan.
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MeSH Terms
Descriptor/Qualifier:
Adenosine Triphosphate / metabolism
Animals
Methylation
Mitochondria, Liver / drug effects
Nitriles / pharmacology*
Oxidative Phosphorylation / drug effects*
Proton-Translocating ATPases / metabolism
Rats
Uncoupling Agents / pharmacology*
Chemical
Reg. No./Substance:
0/Nitriles; 0/Uncoupling Agents; 51601-81-1/SF 6847; 56-65-5/Adenosine Triphosphate; EC 3.6.3.14/Proton-Translocating ATPases

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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