Document Detail


Unique requirements for reactivation of virus-specific memory B lymphocytes.
MedLine Citation:
PMID:  20739675     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Memory B cells (MBCs) are rapidly activated upon Ag re-exposure in vivo, but the precise requirements for this process are still elusive. To address these requirements, T cell-independent reactivation of MBCs against virus-like particles was analyzed. As few as 25 MBCs are sufficient for a measurable Ab response after adoptive transfer. We found that MBCs were reactivated upon antigenic challenge to normal levels after depletion of macrophages, CD11c(+) dendritic cells, and matured follicular dendritic cells. Furthermore, MBC responses were possible in TNF/lymphotoxin α double-deficient mice after partial normalization of lymphoid architecture by means of long-term reconstitution with wild-type bone marrow. Activation did not occur when chimeric mice, which still lack all lymph nodes and Peyer's patches, were splenectomized prior to MBC transfer. Together with our finding that MBC responses are weak when Ag was administered within minutes after adoptive MBC transfer, these results strongly suggest that MBCs have to occupy specific niches within secondary lymphoid tissue to become fully Ag-responsive. We provide clear evidence that MBCs are not preferentially resident within the splenic marginal zones and show that impaired homing to lymphoid follicles resulted in significantly diminished activation, suggesting that reactivation of MBCs occurred inside lymphoid follicles. Furthermore, comparison of virus-specific MBC T cell-independent reactivation versus primary T cell-independent type II B cell activation revealed unique requirements of MBC activation.
Authors:
Florian J Weisel; Uwe K Appelt; Andrea M Schneider; Jasmin U Horlitz; Nico van Rooijen; Heinrich Korner; Michael Mach; Thomas H Winkler
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2010-08-25
Journal Detail:
Title:  Journal of immunology (Baltimore, Md. : 1950)     Volume:  185     ISSN:  1550-6606     ISO Abbreviation:  J. Immunol.     Publication Date:  2010 Oct 
Date Detail:
Created Date:  2010-09-22     Completed Date:  2010-10-19     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  2985117R     Medline TA:  J Immunol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  4011-21     Citation Subset:  AIM; IM    
Affiliation:
Hematopoiesis Unit, Department of Biology, University Erlangen, Erlangen, Germany.
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MeSH Terms
Descriptor/Qualifier:
Adoptive Transfer
Animals
Antibodies, Viral / blood
B-Lymphocytes / immunology*,  virology
Cell Separation
Chemotaxis, Leukocyte / immunology*
Cytomegalovirus / immunology
Enzyme-Linked Immunosorbent Assay
Flow Cytometry
Immunologic Memory / immunology*
Lymphocyte Activation / immunology*
Lymphoid Tissue / cytology,  immunology
Mice
Mice, Inbred C57BL
Mice, Transgenic
Microscopy, Fluorescence
Virion / immunology*
Chemical
Reg. No./Substance:
0/Antibodies, Viral

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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