Document Detail


Uniform yeast cell assembly via microfluidics.
MedLine Citation:
PMID:  22655026     Owner:  NLM     Status:  PubMed-not-MEDLINE    
Abstract/OtherAbstract:
This paper reports the use of microfluidic approaches for the fabrication of yeastosomes (yeast-celloidosomes) based on self-assembly of yeast cells onto liquid-solid or liquid-gas interfaces. Precise control over fluidic flows in droplet- and bubble-forming microfluidic devices allows production of monodispersed, size-selected templates. The general strategy to organize and assemble living cells is to tune electrostatic attractions between the template (gel or gas core) and the cells via surface charging. Layer-by-Layer (LbL) polyelectrolyte deposition was employed to invert or enhance charges of solid surfaces. We demonstrated the ability to produce high-quality, monolayer-shelled yeastosome structures under proper conditions when sufficient electrostatic driving forces are present. The combination of microfluidic fabrication with cell self-assembly enables a versatile platform for designing synthetic hierarchy bio-structures.
Authors:
Ya-Wen Chang; Peng He; Samantha M Marquez; Zhengdong Cheng
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Publication Detail:
Type:  Journal Article     Date:  2012-05-08
Journal Detail:
Title:  Biomicrofluidics     Volume:  6     ISSN:  1932-1058     ISO Abbreviation:  Biomicrofluidics     Publication Date:  2012 Jun 
Date Detail:
Created Date:  2012-06-01     Completed Date:  2012-08-23     Revised Date:  2013-02-26    
Medline Journal Info:
Nlm Unique ID:  101293825     Medline TA:  Biomicrofluidics     Country:  United States    
Other Details:
Languages:  eng     Pagination:  24118-241189     Citation Subset:  -    
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