| Unheated Cannabis sativa extracts and its major compound THC-acid have potential immuno-modulating properties not mediated by CB1 and CB2 receptor coupled pathways. | |
| | |
MedLine Citation:
|
PMID: 16504929 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
There is a great interest in the pharmacological properties of cannabinoid like compounds that are not linked to the adverse effects of Delta(9)-tetrahydrocannabinol (THC), e.g. psychoactive properties. The present paper describes the potential immuno-modulating activity of unheated Cannabis sativa extracts and its main non-psychoactive constituent Delta(9)-tetrahydrocanabinoid acid (THCa). By heating Cannabis extracts, THCa was shown to be converted into THC. Unheated Cannabis extract and THCa were able to inhibit the tumor necrosis factor alpha (TNF-alpha) levels in culture supernatants from U937 macrophages and peripheral blood macrophages after stimulation with LPS in a dose-dependent manner. This inhibition persisted over a longer period of time, whereas after prolonged exposure time THC and heated Cannabis extract tend to induce the TNF-alpha level. Furthermore we demonstrated that THCa and THC show distinct effects on phosphatidylcholine specific phospholipase C (PC-PLC) activity. Unheated Cannabis extract and THCa inhibit the PC-PLC activity in a dose-dependent manner, while THC induced PC-PLC activity at high concentrations. These results suggest that THCa and THC exert their immuno-modulating effects via different metabolic pathways. |
| | |
Authors:
|
Kitty C M Verhoeckx; Henrie A A J Korthout; A P van Meeteren-Kreikamp; Karl A Ehlert; Mei Wang; Jan van der Greef; Richard J T Rodenburg; Renger F Witkamp |
Related Documents
:
|
11199139 - Protective effects of green tea catechins against asbestos-induced cell injury. 16428919 - Ginkgo biloba: evaluation of cyp2c9 drug interactions in vitro and in vivo. 11853179 - Antipruritic effect of cnidii monnieri fructus (fruits of cnidium monnieri cusson). 3011439 - Augmentation of human blood monocyte microbicidal activity by ru 41740, a glycoprotein ... 9648229 - Antimutagenicity of flavones and flavonols to heterocyclic amines by specific and stron... 6360409 - Inhibition of the mutagenicity of bay-region diol-epoxides of polycyclic aromatic hydro... 7895399 - Phospholipase a2 activity in peripheral blood cells of rheumatoid arthritis patients tr... 2073689 - Platelet-activating factor (paf) and paf antagonists in asthma. 22425319 - Platelet tissue factor pathway inhibitor modulates intravascular coagulation. |
Publication Detail:
|
Type: Journal Article Date: 2005-11-07 |
Journal Detail:
|
Title: International immunopharmacology Volume: 6 ISSN: 1567-5769 ISO Abbreviation: Int. Immunopharmacol. Publication Date: 2006 Apr |
Date Detail:
|
Created Date: 2006-02-28 Completed Date: 2006-04-26 Revised Date: 2008-11-21 |
Medline Journal Info:
|
Nlm Unique ID: 100965259 Medline TA: Int Immunopharmacol Country: Netherlands |
Other Details:
|
Languages: eng Pagination: 656-65 Citation Subset: IM |
Affiliation:
|
TNO Pharma, Utrechtseweg 48, PZeist, The Netherlands. Verhoeckx@pharma.tno.nl |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Cannabis
/
chemistry*,
immunology* Cell Line Cells, Cultured Cyclic AMP / pharmacology Dose-Response Relationship, Drug Hot Temperature Humans Immunologic Factors* Macrophage Activation / drug effects Macrophages / drug effects, immunology Plant Extracts / chemistry, pharmacology Receptor, Cannabinoid, CB1 / antagonists & inhibitors, drug effects* Receptor, Cannabinoid, CB2 / antagonists & inhibitors, drug effects* Signal Transduction / drug effects Tetrahydrocannabinol / pharmacology* Tumor Necrosis Factor-alpha / metabolism Type C Phospholipases / metabolism |
| Chemical | |
Reg. No./Substance:
|
0/Immunologic Factors; 0/Plant Extracts; 0/Receptor, Cannabinoid, CB1; 0/Receptor, Cannabinoid, CB2; 0/Tumor Necrosis Factor-alpha; 1972-08-3/Tetrahydrocannabinol; 60-92-4/Cyclic AMP; EC 3.1.4.-/Type C Phospholipases |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: An in vivo model to assess factors that may stimulate the generation of an immune reaction to erythr...
Next Document: Antipsychotic treatment may alter T-helper (TH) 2 arm cytokines.