Document Detail


Unfractionated human marrow cell cryopreservation using dimethylsulfoxide and hydroxyethyl starch.
MedLine Citation:
PMID:  6187518     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Unfractionated bone marrow (BM) cells were cryopreserved in 1- to 2-ml aliquots using a mixture containing both 5% dimethylsulfoxide (DMSO) and 6% hydroxyethyl starch (HES) in an attempt to increase the viable cell yield and reduce the clumping after thawing, observed when 10% DMSO is used alone. Samples thawed after storage for 6 months in the vapor phase of liquid nitrogen, were assayed. Compared to prefreeze values, there was both a greater number of cells that excluded Trypan Blue (50 +/- 12 vs 28 +/- 12%, P less than .01) and a greater CFU-C Recovery (110 +/- 20 vs 89 +/- 35%, P less than .02) for cells in the DMSO/HES mixture, compared to those in 10% DMSO alone. No macroscopic clumping of the thawed cells was observed for those cryopreserved in the mixture in contrast to those in DMSO alone. Freezing was done without a rate-controlled freezing apparatus by simply placing the samples initially into a -80 degrees C freezer, and then later into a liquid nitrogen freezer. Additional samples stored in the DMSO/HES mixture were kept at only -80 degrees C, and when thawed 12 to 16 months later also gave an excellent CFU-C recovery (105 +/- 39% of prefreeze). The DMSO/HES mixture allows for a simplified BM cryopreservation technique that not only assures excellent recovery of CFU-Cs and eliminates clumping upon thawing, but also does not require either the use of a rate-controlled freezer or liquid nitrogen temperatures for storage up to a year.
Authors:
P J Stiff; A J Murgo; C G Zaroulis; M F DeRisi; B D Clarkson
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Publication Detail:
Type:  Comparative Study; In Vitro; Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Cryobiology     Volume:  20     ISSN:  0011-2240     ISO Abbreviation:  Cryobiology     Publication Date:  1983 Feb 
Date Detail:
Created Date:  1983-05-05     Completed Date:  1983-05-05     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  0006252     Medline TA:  Cryobiology     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  17-24     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Bone Marrow Cells*
Cell Survival
Cryoprotective Agents*
Dimethyl Sulfoxide
Freezing*
Hetastarch
Humans
Preservation, Biological / methods*
Time Factors
Grant Support
ID/Acronym/Agency:
A29941-01//PHS HHS; CA 08748/CA/NCI NIH HHS; CA 19117-05/CA/NCI NIH HHS
Chemical
Reg. No./Substance:
0/Cryoprotective Agents; 67-68-5/Dimethyl Sulfoxide; 9005-27-0/Hetastarch

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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