Document Detail

Unfolded Protein Response (UPR) is activated during normal lens development.
MedLine Citation:
PMID:  21044701     Owner:  NLM     Status:  MEDLINE    
The lens of the eye is a transparent structure responsible for focusing light onto the retina. It is composed of two morphologically different cell types, epithelial cells found on the anterior surface and the fiber cells that are continuously formed by the differentiation of epithelial cells at the lens equator. The differentiation of an epithelial precursor cell into a fiber cell is associated with a dramatic increase in membrane protein synthesis. How the terminally differentiating fiber cells cope with the increased demand on the endoplasmic reticulum for this membrane protein synthesis is not known. In the present study, we have found evidence of Unfolded Protein Response (UPR) activation during normal lens development and differentiation in the mouse. The ER-resident chaperones, immunoglobulin heavy chain binding protein (BiP) and protein disulfide isomerase (PDI), were expressed at high levels in the newly forming fiber cells of embryonic lenses. These fiber cells also expressed the UPR-associated molecules; XBP1, ATF6, phospho-PERK and ATF4 during embryogenesis. Moreover, spliced XBP1, cleaved ATF6, and phospho-eIF2α were detected in embryonic mouse lenses suggesting that UPR pathways are active in this tissue. These results propose a role for UPR activation in lens fiber cell differentiation during embryogenesis.
Zeynep Firtina; Melinda K Duncan
Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural     Date:  2010-10-31
Journal Detail:
Title:  Gene expression patterns : GEP     Volume:  11     ISSN:  1872-7298     ISO Abbreviation:  Gene Expr. Patterns     Publication Date:    2011 Jan-Feb
Date Detail:
Created Date:  2011-02-14     Completed Date:  2011-03-24     Revised Date:  2014-09-15    
Medline Journal Info:
Nlm Unique ID:  101167473     Medline TA:  Gene Expr Patterns     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  135-43     Citation Subset:  IM    
Copyright Information:
Copyright © 2010 Elsevier B.V. All rights reserved.
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MeSH Terms
Activating Transcription Factor 6
Cell Differentiation
Embryo, Mammalian / metabolism
Lens, Crystalline / cytology,  embryology*
Signal Transduction
Unfolded Protein Response*
eIF-2 Kinase / metabolism
Grant Support
EY015279/EY/NEI NIH HHS; P20 RR16472/RR/NCRR NIH HHS; R01 EY015279/EY/NEI NIH HHS; R01 EY015279-08/EY/NEI NIH HHS
Reg. No./Substance:
0/Activating Transcription Factor 6; 0/Atf6 protein, mouse; EC 2.7.10.-/PERK kinase; EC Kinase

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