Document Detail


Unexpectedly high genetic variation in large unisexual clumps of the subdioecious plant Honckenya peploides (Caryophyllaceae).
MedLine Citation:
PMID:  20522189     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Honckenya peploides is a subdioecious dune plant that reproduces both sexually and by clonal growth. In northwest Spain this species was found to exhibit an extreme spatial segregation of the sexes, and our objective was to investigate genetic variation in unisexual clumps. Genetic variation was studied in six unisexual clumps of H. peploides, three of them exclusively composed of males and three exclusively female. In total, 193 samples were analysed using isozyme analysis and 80 samples were analysed using two AFLP primer combinations. Both techniques revealed considerably high genetic diversity (average proportion of distinguishable genotypes: 0.22 for isozymes and 0.36 for AFLP; average Simpson's D: 0.65 for isozymes and 0.68 for AFLP). Our results show that, in spite of clonal growth, each unisexual clump consists of different genotypes. Genetic diversity within clumps is similar for both sexual morphs. Reasons for unisexuality of the clumps are discussed.
Authors:
J Sánchez-Vilas; M Philipp; R Retuerto
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Plant biology (Stuttgart, Germany)     Volume:  12     ISSN:  1438-8677     ISO Abbreviation:  Plant Biol (Stuttg)     Publication Date:  2010 May 
Date Detail:
Created Date:  2010-06-04     Completed Date:  2010-08-18     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101148926     Medline TA:  Plant Biol (Stuttg)     Country:  England    
Other Details:
Languages:  eng     Pagination:  518-25     Citation Subset:  IM    
Affiliation:
Area de Ecología, Facultad de Biología, Avda. Lope Gómez de Marzoa s/n, Universidad de Santiago de Compostela, Santiago de Compostela, Spain. julia.sanchezvilas@plants.ox.ac.uk
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MeSH Terms
Descriptor/Qualifier:
Amplified Fragment Length Polymorphism Analysis
Caryophyllaceae / enzymology,  genetics*
DNA, Plant / genetics
Genetic Variation*
Genotype
Isoenzymes / analysis
Sequence Analysis, DNA
Spain
Chemical
Reg. No./Substance:
0/DNA, Plant; 0/Isoenzymes

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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