Document Detail

Unexpected limitations in the use of commercial antiglobulin reagents.
MedLine Citation:
PMID:  1082669     Owner:  NLM     Status:  MEDLINE    
The majority of antiglobulin sera used in blood banks in the USA are commercially prepared immune rabbit sera, designed to be reactive only with human red blood cells sensitized with immunoglobulins or complement components. Current manufacturing methods result in a product that gives reliable specific reactions provided that the test red blood cells are normal, particularly with respect to sialic acid levels. This report describes our findings of false positive antiglobulin tests caused by incompletely absorbed antiglobulin reagents when cells with low sialic acid levels were tested. An evaluation of nine commercially prepared antiglobulin reagents revealed, in many of them, the presence of anti-species antibody, anti-T, and anti-Tn. Blood bank personnel must be aware of these characteristics especially when testing either enzyme premodified or polyagglutinable cells. The use of incompletely absorbed reagents might account for positive direct antiglobulin tests that are encountered occasionally in apparently normal healthy individuals. Furthermore, recent protocols advocating the use of trypsinized cells for the evaluation of anti-C3d activity of antiglobulin sera are invalid if the reagent is inadequately absorbed of anti-species agglutinins.
M L Beck; B Hicklin; S R Pierce
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Transfusion     Volume:  16     ISSN:  0041-1132     ISO Abbreviation:  Transfusion     Publication Date:    1976 Jan-Feb
Date Detail:
Created Date:  1976-04-09     Completed Date:  1976-04-09     Revised Date:  2004-11-17    
Medline Journal Info:
Nlm Unique ID:  0417360     Medline TA:  Transfusion     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  71-5     Citation Subset:  IM    
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MeSH Terms
Antibodies, Anti-Idiotypic / standards*
Blood Banks*
Blood Group Antigens
Coombs' Test
Erythrocytes / analysis,  immunology
False Positive Reactions
Sialic Acids / blood
Species Specificity
Reg. No./Substance:
0/Agglutinins; 0/Antibodies, Anti-Idiotypic; 0/Blood Group Antigens; 0/Sialic Acids

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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