Document Detail


Unexpected detrimental effect of Insulin like growth factor-1 on bovine oocyte developmental competence under heat stress.
MedLine Citation:
PMID:  19915974     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
PURPOSE: To investigate the effects of Insulin like growth factor-1 (IGF-1) on bovine oocyte developmental competence under heat stress.
METHODS: In Experiment 1, bovine cumulus-oocyte complexes (COCs) were cultured at 38.5 or 41 degrees C for the first 12 h of maturation in the presence of either 100 ng/ml human recombinant (hr)-IGF-1 or acetic acid. In Experiment 2, COCs were cultured in 38.5 or 41 degrees C for the first 12 h of maturation in the presence either of 100 ng/ml hr-IGF-1 or acetic acid. After fertilization, putative zygotes were cultured for 8 days.
RESULTS: In experiment 1, addition of rh-IGF-1 to maturation medium at 38.5 degrees C significantly increased the proportion of M II oocytes and decreased the percentage of TUNEL-positive oocytes compared to the other groups. However, addition of rh-IGF-1 to maturation medium under heat stress increased the percentage of TUNEL-positive oocytes. In experiment 2, addition of rh-IGF-1 under heat sress did not affect cleavage rate, whereas, blastocyst formation rate decreased in heat-stressed and heat-stressed plus rh-IGF-1 groups. Similarly, The number of trophectoderm cells and total cell number were decreased in heat-stressed and heat-stressed plus rh-IGF-1 groups and the percentage of TUNEL-positive nuclei were increased in heat-stressed and heat-stressed plus rh-IGF-1 groups compared to the other groups.
CONCLUSION: The results of the present study demonstrate that IGF-1 decreases oocyte developmental competence and total cell number and increases TUNEL-positive nuclei at heat stress condition. These unexpected results of IGF-1 during maturation period under heat stress condition warrant further optimizations and investigations.
Authors:
Mahdi Zhandi; Armin Towhidi; Mohammad Hossein Nasr-Esfahani; Poopak Eftekhari-Yazdi; Ahmad Zare-Shahneh
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2009-11-14
Journal Detail:
Title:  Journal of assisted reproduction and genetics     Volume:  26     ISSN:  1573-7330     ISO Abbreviation:  J. Assist. Reprod. Genet.     Publication Date:    2009 Nov-Dec
Date Detail:
Created Date:  2010-01-21     Completed Date:  2010-04-08     Revised Date:  2013-05-31    
Medline Journal Info:
Nlm Unique ID:  9206495     Medline TA:  J Assist Reprod Genet     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  605-11     Citation Subset:  IM    
Affiliation:
Department of Animal Science, University College of Agriculture and Natural Resources, University of Tehran, POBox#4111, Karaj, Iran. mzhandi@ut.ac.ir
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MeSH Terms
Descriptor/Qualifier:
Animals
Apoptosis / physiology
Cattle / physiology*
DNA Fragmentation
Female
Heat Stress Disorders / pathology,  veterinary*
In Situ Nick-End Labeling / veterinary
Insulin-Like Growth Factor I / pharmacology*
Male
Oocytes / drug effects*,  growth & development
Pregnancy
Chemical
Reg. No./Substance:
67763-96-6/Insulin-Like Growth Factor I
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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