Document Detail


Unequivocal demonstration that malondialdehyde is a mutagen.
MedLine Citation:
PMID:  6339098     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Malondialdehyde (MDA), a product of lipid peroxidation and prostaglandin biosynthesis, has been reported to be mutagenic and carcinogenic. Recent evidence suggests, however, that strongly mutagenic impurities are generated during the preparation of MDA that may contribute to the observed biological activity. Since MDA is widely produced in animal tissue it is important to establish whether it is actually mutagenic and carcinogenic. We have utilized three complementary methods for the preparation of highly purified MDA for biological testing. These are chromatographic purification of the sodium salt of MDA, sublimation of the free acid of MDA, and basic hydrolysis of beta-(p-nitrophenoxy)acrolein. The latter is a unique method that we have developed specifically to generate MDA under non-acidic conditions where it is stable. MDA prepared by each method induced approximately 5 revertants/mumol in Salmonella typhimurium his D 3052. This unequivocally demonstrates that MDA is a weak mutagen.
Authors:
A K Basu; L J Marnett
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Carcinogenesis     Volume:  4     ISSN:  0143-3334     ISO Abbreviation:  Carcinogenesis     Publication Date:  1983  
Date Detail:
Created Date:  1983-05-05     Completed Date:  1983-05-05     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  8008055     Medline TA:  Carcinogenesis     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  331-3     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Chemical Phenomena
Chemistry
Hydrolysis
Malonates / toxicity*
Malondialdehyde / toxicity*
Mutagenicity Tests
Mutagens*
Mutation*
Salmonella typhimurium / drug effects
Grant Support
ID/Acronym/Agency:
CA 22206/CA/NCI NIH HHS
Chemical
Reg. No./Substance:
0/Malonates; 0/Mutagens; 542-78-9/Malondialdehyde

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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