Document Detail


Undifferentiated U937 cells transfected with chemoattractant receptors: a model system to investigate chemotactic mechanisms and receptor structure/function relationships.
MedLine Citation:
PMID:  9060456     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Transfection of either the C5a receptor or the formyl peptide receptor into undifferentiated U937 cells generated continuously growing cell lines that stably expressed these receptors. The transfected cells displayed significant numbers of cell surface receptors that had ligand binding properties similar to fully differentiated U937 cells. Undifferentiated transfected U937 cells were capable of a ligand-specific calcium flux and showed migratory responses that were qualitatively and quantitatively similar to differentiated cells and were specific for each chemoattractant. Moreover, the chemotactic response could be desensitized by preincubating the cells in a high concentration of ligand and could be blocked by pertussis toxin. These results demonstrate that undifferentiated U937 cells possess the subcellular signaling apparatus and machinery necessary to generate a motile response and that the only missing component for chemotaxis is expression of a chemoattractant receptor. In addition, the results demonstrate that undifferentiated U937 cells transfected with chemoattractant receptors provide a defined model system to study receptor structure/function relationships and may be used to investigate receptor-mediated chemotactic responses in a relevant human myeloid cell.
Authors:
R R Kew; T Peng; S J DiMartino; D Madhavan; S J Weinman; D Cheng; E R Prossnitz
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Journal of leukocyte biology     Volume:  61     ISSN:  0741-5400     ISO Abbreviation:  J. Leukoc. Biol.     Publication Date:  1997 Mar 
Date Detail:
Created Date:  1997-04-03     Completed Date:  1997-04-03     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  8405628     Medline TA:  J Leukoc Biol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  329-37     Citation Subset:  IM    
Affiliation:
Department of Pathology, State University of New York at Stony Brook, USA.
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MeSH Terms
Descriptor/Qualifier:
Antigens, CD / genetics,  metabolism*,  physiology
Calcium / metabolism*
Chemotaxis*
Humans
N-Formylmethionine Leucyl-Phenylalanine
Receptor, Anaphylatoxin C5a
Receptors, Complement / genetics,  metabolism*,  physiology
Receptors, Formyl Peptide
Receptors, Immunologic / genetics,  metabolism*,  physiology
Receptors, Peptide / genetics,  metabolism*,  physiology
Signal Transduction / physiology*
Transfection
Grant Support
ID/Acronym/Agency:
AI33503/AI/NIAID NIH HHS; AI36357/AI/NIAID NIH HHS
Chemical
Reg. No./Substance:
0/Antigens, CD; 0/Receptor, Anaphylatoxin C5a; 0/Receptors, Complement; 0/Receptors, Formyl Peptide; 0/Receptors, Immunologic; 0/Receptors, Peptide; 59880-97-6/N-Formylmethionine Leucyl-Phenylalanine; 7440-70-2/Calcium

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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