| Undetectable apolipoprotein A-I gene expression suggests an unusual mechanism of dietary lipid mobilisation in the intestine of Cyprinus carpio. | |
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MedLine Citation:
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PMID: 15781899 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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High density lipoprotein (HDL) has been shown to play an important role in the dietary lipid mobilisation in the carp. In spite of this, previous studies have failed to demonstrate the synthesis of the major protein component of HDL, apolipoprotein A-I (apoA-I), in the proximal intestine of the carp. Therefore, the aim of the present study was to evaluate the expression of apoA-I throughout the entire intestine. Curiously, no transcription of the apoA-I gene could be detected either by northern blot or RT-PCR assays in the intestinal mucosa, in clear contrast with the abundant cytosolic immunoreactive apoA-I detected in almost all intestinal segments, which suggests a different origin for this protein. In addition, the detection of specific, but low affinity, binding sites for apoA-I in the carp intestinal brush-border membranes (BBM), and the strong interaction with BBM, which is highly dependent on temperature, points to an important contribution of membrane lipids in apoA-I binding to the intestinal mucosa. This idea was reinforced by the ability of carp apoA-I to associate with multilamellar phospholipid vesicles. |
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Authors:
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Margarita I Concha; Rodrigo López; Julieta Villanueva; Nadya Báez; Rodolfo Amthauer |
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Publication Detail:
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Type: Comparative Study; Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: The Journal of experimental biology Volume: 208 ISSN: 0022-0949 ISO Abbreviation: J. Exp. Biol. Publication Date: 2005 Apr |
Date Detail:
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Created Date: 2005-03-22 Completed Date: 2005-06-07 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 0243705 Medline TA: J Exp Biol Country: England |
Other Details:
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Languages: eng Pagination: 1393-9 Citation Subset: IM |
Affiliation:
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Instituto de Bioquímica, Facultad de Ciencias, Universidad Austral de Chile, Campus Isla Teja, Valdivia, Chile. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Apolipoprotein A-I / metabolism* Blotting, Northern Carps / metabolism* DNA Primers Dietary Fats / metabolism* Dimyristoylphosphatidylcholine Gene Expression Regulation* Immunohistochemistry Intestines / metabolism* Microvilli / metabolism Reverse Transcriptase Polymerase Chain Reaction Spectrophotometry |
| Chemical | |
Reg. No./Substance:
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0/Apolipoprotein A-I; 0/DNA Primers; 0/Dietary Fats; 13699-48-4/Dimyristoylphosphatidylcholine |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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