Document Detail


Understanding resistant germplasm-induced virulence variation through analysis of proteomics and suppression subtractive hybridization in a maize pathogen Curvularia lunata.
MedLine Citation:
PMID:  23044763     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
Curvularia lunata is an important pathogen causing Curvularia leaf spot in maize. Significant pathogenic variation has been found in Curvularia lunata. To better understand the mechanism of this phenomenon, we consecutively put the selective pressures of resistant maize population on Curvularia lunata strain WS18 (low virulence) artificially. As a result, the virulence of this strain was significantly enhanced. Using 2-DE, 12 up-regulated and 4 down-regulated proteins were identified in virulence-increased strain compared to WS18. Our analysis revealed that melanin synthesis-related proteins (Brn1, Brn2 and SCD) and stress tolerance-related proteins (HSP 70) directly involved in the potential virulence growth as crucial markers or factors in C. lunata. To validate 2-DE results and screen differential genes at mRNA level, we constructed a subtracted cDNA library (Tester: virulence-increased strain; Driver: WS18). A total of 188 unigenes were obtained by this way, of which 14 were indicators for the evolution of pathogen virulence. Brn1 and hsp genes exhibited similar expression patterns corresponding to proteins detected by 2-DE. Overall, our results indicated that differential proteins or genes, being involved with melanin synthesis or tolerance response to stress, could be considered as hallmarks of virulence increase in C. lunata.
Authors:
Shigang Gao; Tong Liu; Yingying Li; Qiong Wu; Kehe Fu; Jie Chen
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-10-9
Journal Detail:
Title:  Proteomics     Volume:  -     ISSN:  1615-9861     ISO Abbreviation:  Proteomics     Publication Date:  2012 Oct 
Date Detail:
Created Date:  2012-10-9     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101092707     Medline TA:  Proteomics     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Copyright Information:
© 2012 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.
Affiliation:
School of Agriculture and Biology, Shanghai Jiao Tong University, 800 Dongchuan Road, Shanghai, 200240, P. R. China.
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