Document Detail


Understanding enzyme immobilisation.
MedLine Citation:
PMID:  19169460     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Enzymes are versatile catalysts in the laboratory and on an industrial scale. To broaden their applicability in the laboratory and to ensure their (re)use in manufacturing the stability of enzymes can often require improvement. Immobilisation can address the issue of enzymatic instability. Immobilisation can also help to enable the employment of enzymes in different solvents, at extremes of pH and temperature and exceptionally high substrate concentrations. At the same time substrate-specificity, enantioselectivity and reactivity can be modified. However, most often the molecular and physical-chemical bases of these phenomena have not been elucidated yet. This tutorial review focuses on the understanding of enzyme immobilisation.
Authors:
Ulf Hanefeld; Lucia Gardossi; Edmond Magner
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Review     Date:  2008-12-09
Journal Detail:
Title:  Chemical Society reviews     Volume:  38     ISSN:  0306-0012     ISO Abbreviation:  Chem Soc Rev     Publication Date:  2009 Feb 
Date Detail:
Created Date:  2009-01-26     Completed Date:  2009-03-20     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0335405     Medline TA:  Chem Soc Rev     Country:  England    
Other Details:
Languages:  eng     Pagination:  453-68     Citation Subset:  IM    
Affiliation:
Gebouw voor Scheikunde, Afdeling Biotechnologie, Technische Universiteit Delft, Julianalaan 136, Delft, 2628 BL, The Netherlands. u.hanefeld@tudelft.nl
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MeSH Terms
Descriptor/Qualifier:
Adsorption
Enzymes, Immobilized / chemistry*,  metabolism
Hydrogen Bonding
Ions / chemistry
Lipase / chemistry,  metabolism
Stereoisomerism
Chemical
Reg. No./Substance:
0/Enzymes, Immobilized; 0/Ions; EC 3.1.1.3/Lipase

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