| Under-filled blood collection tubes containing K2EDTA as anticoagulant are acceptable for automated complete blood counts, white blood cell differential, and reticulocyte count. | |
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MedLine Citation:
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PMID: 20041968 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Current laboratory standards from Clinical Laboratory Standards Institute (CLSI) and manufacturer's (Becton Dickinson) data indicate that under-filling K(2)EDTA blood collection tubes can result in erroneous hematology values. To accommodate under-filled tubes and reduce collection volumes while optimizing our automation, we explored the acceptable limit of under-filled tubes for hematology values. We collected 8.0 ml of blood from 30 normal adult volunteers. Each donation was aliquoted in the following volumes: 4.0, 2.0, 1.0, 0.5 ml x 2. These samples were analyzed within 1 h of blood collection on Sysmex XE-2100 (Sysmex America Inc., Mundelein, IL, USA) for complete blood count, reticulocyte, and white blood cell differentials. Results of the under-filled tubes were compared to those of the standard volume. The Deming regression analysis show excellent correlation for all parameters between each under-filled blood collection volume compared to a standard 4 ml volume. The Bland and Altman analysis shows good agreement between both 1.0 and 2.0 ml compared to a 4.0 ml volume. The 0.5 ml compared to a 4.0 ml volume, however, shows increased variation on many parameters. In addition all three collection volumes show negative bias compared to the standard volume for platelet count, but the difference is considered insignificant with a percent difference of 5.5%, 3.2%, and 1.5% for 0.5, 1.0, and 2.0 ml collection volume respectively. Finally for 0.5 ml collection volume we noticed a low level of false positive flagging rate for white blood cell. Acceptable complete blood count values of under-filled powdered K(2)EDTA tubes can be obtained with as little as 1.0 ml of blood. |
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Authors:
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M Xu; V A Robbe; R M Jack; J C Rutledge |
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Publication Detail:
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Type: Journal Article Date: 2009-12-23 |
Journal Detail:
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Title: International journal of laboratory hematology Volume: 32 ISSN: 1751-553X ISO Abbreviation: Int J Lab Hematol Publication Date: 2010 Oct |
Date Detail:
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Created Date: 2010-08-24 Completed Date: 2010-12-20 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 101300213 Medline TA: Int J Lab Hematol Country: England |
Other Details:
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Languages: eng Pagination: 491-7 Citation Subset: IM |
Affiliation:
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Department of Laboratories, Seattle Children's Hospital, Seattle, WA 98105, USA. min.xu@seattlechildrens.org |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Adult Anticoagulants Blood Cell Count / standards* Blood Specimen Collection / instrumentation*, standards Edetic Acid Female Humans Leukocyte Count / instrumentation, standards* Reticulocyte Count / instrumentation, standards* |
| Chemical | |
Reg. No./Substance:
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0/Anticoagulants; 60-00-4/Edetic Acid |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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