| Uncoupling protein mRNA, mitochondrial GTP-binding, and T4 5'-deiodinase activity of brown adipose tissue in Daurian ground squirrel during hibernation and arousal. | |
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MedLine Citation:
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PMID: 10400495 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The mRNA level of uncoupling protein (UCP) specific for brown adipose tissue (BAT) in Daurian ground squirrel, was detected by using a [32P]-labeled oligonucleotide probe. The UCP concentration in mitochondria was indirectly determined by titration with its specific ligand [H3]-labeled GTP. Type II T4 5'-deiodinase of BAT was assayed concomitantly. We found two species of mRNA for UCP with lengths of about 1.9 and 1.5 kb, respectively, both occurring in almost the same concentration. UCP mRNA content was elevated significantly during hibernation, but the UCP concentration did not change compared with that of nonhibernating controls kept at room temperature. When hibernating squirrels were aroused, the UCP mRNA remained at the elevated level as during hibernation, but the UCP concentration increased in comparison with that of nonhibernating controls or during hibernating. Changes in T4 5'-deiodinase activity in BAT were similar to the variations of the UCP mRNA level. These results suggest that the activation of T4 5'-deiodinase in BAT may be an important factor for the up-regulation and maintenance of UCP mRNA content needed for the synthesis of sufficient UCP to acquire the thermogenic capacity for arousal from hibernation. |
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Authors:
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X T Liu; Q S Lin; Q F Li; C X Huang; R Y Sun |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Comparative biochemistry and physiology. Part A, Molecular & integrative physiology Volume: 120 ISSN: 1095-6433 ISO Abbreviation: Comp. Biochem. Physiol., Part A Mol. Integr. Physiol. Publication Date: 1998 Aug |
Date Detail:
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Created Date: 1999-07-08 Completed Date: 1999-07-08 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 9806096 Medline TA: Comp Biochem Physiol A Mol Integr Physiol Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 745-52 Citation Subset: IM; S |
Affiliation:
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Department of Biology, Beijing Normal University, People's Republic of China. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Adipose Tissue
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metabolism* Analysis of Variance Animals Arousal / physiology* Body Weight / physiology GTP-Binding Proteins / metabolism* Hibernation / physiology* Iodide Peroxidase / metabolism* Mitochondria / metabolism* RNA, Messenger / metabolism* Sciuridae |
| Chemical | |
Reg. No./Substance:
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0/RNA, Messenger; EC 1.11.1.8/Iodide Peroxidase; EC 3.6.1.-/GTP-Binding Proteins |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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