Document Detail


Unaltered pancreatic islet blood perfusion in islet amyloid polypeptide-deficient mice.
MedLine Citation:
PMID:  11751075     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
OBJECTIVE: Several biological activities have been ascribed to islet amyloid polypeptide (IAPP). However, their physiological relevance remains unclear. Previous studies in rats with exogenous administration of IAPP suggest that the peptide may increase splanchnic vascular resistance and redistribute the blood flow within the pancreas to the islets. In this study, the use of IAPP-deficient mice allowed us to evaluate possible effects of the lack of IAPP on splanchnic blood perfusion and we could thereby circumvent the potentially pharmacological actions of exogenously administered IAPP. DESIGN: Regional splanchnic blood flow was measured after exogenous administration of IAPP and in IAPP-deficient mice. METHODS: Blood flow values were determined using a non-radioactive microsphere technique in anesthetized animals. RESULTS: No differences in whole pancreatic blood flow or islet blood flow could be detected in IAPP-deficient mice when compared with control mice; neither did IAPP deficiency affect the glucose-induced increase in islet blood flow. Duodenal, ileal and colonic blood flows were similar in IAPP-deficient and control mice. Exogenous administration of IAPP selectively increased islet blood flow in wild-type control mice. CONCLUSIONS: The present findings in the IAPP-deficient mice suggest that the vascular effects seen in the islets after exogenous administration of IAPP to normal mice reflect pharmacological, rather than physiological effects of the peptide. We conclude that the lack of endogenous IAPP within the splanchnic vascular system does not alter the blood perfusion of pancreatic islets or other splanchnic organs.
Authors:
Per-Ola Carlsson; Ella Karlsson; Hindrik Mulder; Samuel Gebre-Medhin
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  European journal of endocrinology / European Federation of Endocrine Societies     Volume:  146     ISSN:  0804-4643     ISO Abbreviation:  Eur. J. Endocrinol.     Publication Date:  2002 Jan 
Date Detail:
Created Date:  2001-12-25     Completed Date:  2002-02-25     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  9423848     Medline TA:  Eur J Endocrinol     Country:  England    
Other Details:
Languages:  eng     Pagination:  107-12     Citation Subset:  IM    
Affiliation:
Department of Medical Cell Biology, Biomedical Center, Box 571, Uppsala University, SE-751 23 Uppsala, Sweden. Per-Ola.Carlsson@medcellbiol.uu.se
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MeSH Terms
Descriptor/Qualifier:
Amyloid / deficiency*,  genetics*,  pharmacology
Animals
Blood Glucose / metabolism
Blood Pressure / physiology
Body Weight / physiology
Injections, Intravenous
Insulin / blood
Intestines / blood supply
Islets of Langerhans / blood supply*
Male
Mice
Mice, Inbred C57BL
Mice, Knockout
Regional Blood Flow / physiology
Splanchnic Circulation / physiology*
Chemical
Reg. No./Substance:
0/Amyloid; 0/Blood Glucose; 11061-68-0/Insulin

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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