| Ultraviolet-induced inhibition of ribosomal RNA synthesis in yeast strains differing in radiation sensitivities. | |
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MedLine Citation:
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PMID: 793628 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The ultraviolet-induced inhibition of rRNA synthesis has been measured during the first hour after irradiation for stationary yeast cells differing in radiation sensitivity. rRNA was isolated and separated on an agarose-polyacrylamide gel. The wild type and a mutant which is possibly defective in recombinational repair show a sigmoidal inhibition curve, an excision-deficient mutant shows an exponential one. From these curves it is deduced that a pyrimidine dimer acts as a transcription terminating lesion as was shown for bacteria. During the first hour after irradiation the excision repair system decreases the number of transcription terminating lesions by 22% in the wild type and 25% in the mutant defective in recombinational repair. An approximation of the repair efficiency gives a value of 7500-10 000 transcription terminating lesions per cell being removed during the first hour after irradiation by excision. Ultraviolet-induced lesions of this kind can partially be removed by photoreactivation. The inhibition coefficients are the same for 26 S and 18 S rRNA in stationary cells, whereas exponentially growing cells show different inhibition coefficients for 26 S and 18 S rRNA leading to the suggestion that the processing of the ribosomal precursor RNA is different in stationary and exponentially growing cells. |
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Authors:
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H Koch; H Waller; J Kiefer |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Biochimica et biophysica acta Volume: 454 ISSN: 0006-3002 ISO Abbreviation: Biochim. Biophys. Acta Publication Date: 1976 Dec |
Date Detail:
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Created Date: 1977-02-26 Completed Date: 1977-02-26 Revised Date: 2000-12-18 |
Medline Journal Info:
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Nlm Unique ID: 0217513 Medline TA: Biochim Biophys Acta Country: NETHERLANDS |
Other Details:
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Languages: eng Pagination: 436-46 Citation Subset: IM |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Cell Division Dose-Response Relationship, Radiation Genetic Code Mutation RNA, Ribosomal / biosynthesis*, radiation effects Saccharomyces cerevisiae / metabolism, radiation effects* Species Specificity Transcription, Genetic / radiation effects* Ultraviolet Rays* |
| Chemical | |
Reg. No./Substance:
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0/RNA, Ribosomal |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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