Document Detail


Ultraviolet-induced autofluorescence characterization of normal and tumoral esophageal epithelium cells with quantitation of NAD(P)H.
MedLine Citation:
PMID:  16685326     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Cellular autofluorescence was characterized in normal human esophageal cells and in malignant esophageal epithelial cells. The study was performed under excitation at 351 nm where the cell fluorescence is mainly due to the reduced pyridine nucleotides (NAD(P)H) with a very small contribution from the oxidized flavins (FMN, FAD) or lipopigments. The autofluorescence emission of squamous cell carcinoma, adenocarcinoma on Barrett's mucosa and normal cells was characterized by microspectrofluorimetry on monolayers and by spectrofluorimetry on cell suspensions. The relative contribution of each fluorophore to the fluorescence emission of the different cell types was evaluated by a curve-fitting analysis. A statistically highly significant difference was observed between the average intensity of the raw spectra of the different cell types. Tumoral cells had a fluorescence intensity approximately twice as high as that of normal cells. The results of the NAD(P)H quantitation analyzed by microspectrofluorimetry on single living cells and spectrofluorimetry on cell suspensions were consistent with those obtained by biochemical cycling assays, showing that the amount of intracellular NAD(P)H is higher in tumoral cells than in normal cells. Bound NAD(P)H concentration was found to be quite stable whatever the cell type while the amount of free NAD(P)H showed a very important increase in tumoral cells.
Authors:
Sandrine Villette; Sophie Pigaglio-Deshayes; Christine Vever-Bizet; Pierre Validire; Geneviève Bourg-Heckly
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Publication Detail:
Type:  Journal Article     Date:  2006-03-21
Journal Detail:
Title:  Photochemical & photobiological sciences : Official journal of the European Photochemistry Association and the European Society for Photobiology     Volume:  5     ISSN:  1474-905X     ISO Abbreviation:  Photochem. Photobiol. Sci.     Publication Date:  2006 May 
Date Detail:
Created Date:  2006-05-10     Completed Date:  2007-08-23     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101124451     Medline TA:  Photochem Photobiol Sci     Country:  England    
Other Details:
Languages:  eng     Pagination:  483-92     Citation Subset:  IM    
Affiliation:
Laboratoire de Physicochimie Biomoléculaire et Cellulaire, Université Pierre et Marie Curie, CNRS UMR 7033, Paris, France.
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MeSH Terms
Descriptor/Qualifier:
Barrett Esophagus / pathology
Carcinoma, Squamous Cell / chemistry*,  pathology*
Cell Line, Tumor
Esophageal Neoplasms / chemistry*,  pathology*
Flavin Mononucleotide / analysis
Flavin-Adenine Dinucleotide / analysis
Flow Cytometry
Fluorescence
Humans
NADP / analysis*
Spectrometry, Fluorescence
Ultraviolet Rays
Chemical
Reg. No./Substance:
146-14-5/Flavin-Adenine Dinucleotide; 146-17-8/Flavin Mononucleotide; 53-59-8/NADP

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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