Document Detail

Ultraviolet-inactivation of conidia from heterokaryons of Neurospora crassa containing uv-sensitive mutations.
MedLine Citation:
PMID:  123634     Owner:  NLM     Status:  MEDLINE    
The effect of three UV-sensitive mutations of Neurospora crassa, upr-I, uvs-4 and uvs-6, on the ultraviolet-inactivation of conidia from two-component heterokaryons was investigated. In two-component heterokaryons with wild-type sensitivity to radiation inactivation, all three conidial fractions exhibited similar ultraviolet-inactivation curves. Each UV-sensitive mutation studied uniquely modified the ultraviolet-inactivation curves of conidia from two-component heterokaryons. In heterokaryons heterokaryotic for upr-I, the upr-I mutation was recessive and the repair function determined by the wild type allele was functional to some degree in homokaryotic upr-I conidia. All three conidial fractions of heterokaryons containing upr-I in both components showed increased sensitivity to ultraviolet light. The uvs-4 mutation was recessive and resulted in conidia with increased UV-sensitivity only when included in both components of a heterokaryon. Homokaryotic uvs-4 conidia, which arose from heterokaryons containing both uvs-4 and wild-type components, exhibited wild-type survival. Therefore, as with upr-I, there was a carryover the repair capability to conidia which were genetically UV-sensitive. The uvs-6 mutation, when included in one component of a two-component heterokaryon, resulted in increased UV-sensitivity of both heterokaryotic and homokaryotic uvs-6 conidia. When both components contained uvs-6, the UV-sensitivity of all three conidial fractions was increased and all showed similar inactivation curves. Thus, as with upr-I and uvs-4, there was a carryover of the wild-type repair capability to genetically uvs-6 conidia. Heterokaryon tests for complementation between two non-allelic UV-sensitive mutations showed that in heterokaryotic conidia, complete complementation occurred between upr-I and uvs-4.
M D Shelby; F J De Serres; G J Stine
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Mutation research     Volume:  27     ISSN:  0027-5107     ISO Abbreviation:  Mutat. Res.     Publication Date:  1975 Jan 
Date Detail:
Created Date:  1975-06-21     Completed Date:  1975-06-21     Revised Date:  2000-12-18    
Medline Journal Info:
Nlm Unique ID:  0400763     Medline TA:  Mutat Res     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  45-58     Citation Subset:  IM    
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MeSH Terms
Cell Nucleus / radiation effects
DNA Repair
Genes, Recessive
Genetic Complementation Test
Linkage (Genetics)
Neurospora / radiation effects*
Neurospora crassa / radiation effects*,  ultrastructure
Radiation Genetics*
Spores, Fungal / radiation effects,  ultrastructure
Ultraviolet Rays*

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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