| Ultraviolet-inactivation of conidia from heterokaryons of Neurospora crassa containing uv-sensitive mutations. | |
| | |
MedLine Citation:
|
PMID: 123634 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
The effect of three UV-sensitive mutations of Neurospora crassa, upr-I, uvs-4 and uvs-6, on the ultraviolet-inactivation of conidia from two-component heterokaryons was investigated. In two-component heterokaryons with wild-type sensitivity to radiation inactivation, all three conidial fractions exhibited similar ultraviolet-inactivation curves. Each UV-sensitive mutation studied uniquely modified the ultraviolet-inactivation curves of conidia from two-component heterokaryons. In heterokaryons heterokaryotic for upr-I, the upr-I mutation was recessive and the repair function determined by the wild type allele was functional to some degree in homokaryotic upr-I conidia. All three conidial fractions of heterokaryons containing upr-I in both components showed increased sensitivity to ultraviolet light. The uvs-4 mutation was recessive and resulted in conidia with increased UV-sensitivity only when included in both components of a heterokaryon. Homokaryotic uvs-4 conidia, which arose from heterokaryons containing both uvs-4 and wild-type components, exhibited wild-type survival. Therefore, as with upr-I, there was a carryover the repair capability to conidia which were genetically UV-sensitive. The uvs-6 mutation, when included in one component of a two-component heterokaryon, resulted in increased UV-sensitivity of both heterokaryotic and homokaryotic uvs-6 conidia. When both components contained uvs-6, the UV-sensitivity of all three conidial fractions was increased and all showed similar inactivation curves. Thus, as with upr-I and uvs-4, there was a carryover of the wild-type repair capability to genetically uvs-6 conidia. Heterokaryon tests for complementation between two non-allelic UV-sensitive mutations showed that in heterokaryotic conidia, complete complementation occurred between upr-I and uvs-4. |
| | |
Authors:
|
M D Shelby; F J De Serres; G J Stine |
Related Documents
:
|
15509584 - Ring1b-mediated h2a ubiquitination associates with inactive x chromosomes and is involv... 8592334 - Gonadal mosaicism for incontinentia pigmenti in a healthy male. 8205614 - Imprinting and x chromosome counting mechanisms determine xist expression in early mous... 9933914 - A novel method for hybridization of saccharomyces species without genetic markers. 18854114 - Short communication: y chromosome microchimerism in female peripheral blood. 2438094 - Correlation of pachytene chromomeres and metaphase bands of human chromosomes, and dist... |
Publication Detail:
|
Type: Journal Article |
Journal Detail:
|
Title: Mutation research Volume: 27 ISSN: 0027-5107 ISO Abbreviation: Mutat. Res. Publication Date: 1975 Jan |
Date Detail:
|
Created Date: 1975-06-21 Completed Date: 1975-06-21 Revised Date: 2000-12-18 |
Medline Journal Info:
|
Nlm Unique ID: 0400763 Medline TA: Mutat Res Country: NETHERLANDS |
Other Details:
|
Languages: eng Pagination: 45-58 Citation Subset: IM |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Cell Nucleus
/
radiation effects DNA Repair Genes, Recessive Genetic Complementation Test Genotype Heterozygote Homozygote Linkage (Genetics) Mutation* Neurospora / radiation effects* Neurospora crassa / radiation effects*, ultrastructure Radiation Genetics* Spores, Fungal / radiation effects, ultrastructure Ultraviolet Rays* |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Quantitative distribution of the protein fractions in human parotid saliva before and after gustator...
Next Document: Laparoscopy: routine pneumoperitoneum via the posterior fornix.