| Ultraviolet hypermutability of a shuttle vector propagated in xeroderma pigmentosum variant cells. | |
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MedLine Citation:
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PMID: 8228338 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Patients with the variant form of xeroderma pigmentosum (XP) have clinical XP including a high frequency of skin cancer but, in contrast to the other forms of XP, have normal post-ultraviolet (UV) DNA excision repair and nearly normal post-UV survival. However, like excision repair-deficient XP cells, the XP variant cells are UV hypermutable. We used a UV-treated plasmid shuttle vector, pZ189, to examine the DNA repair defect in lymphoblastoid cells from an XP variant patient, XPPHBE, and a normal control. Plasmid repair, mutagenesis, and replication occur within transfected cells in a process dependent on the cells' repair capacity. With the XP variant cells post-UV, plasmid survival was normal with but there was an abnormally increased post-UV plasmid mutation frequency. Sequence analysis of the mutated plasmids revealed an increased frequency of plasmids with single base substitution mutations with the XP variant cells. As in earlier studies with UV mutagenesis, there was a predominance of G:C-->A:T base substitution mutations with plasmids recovered from both cell lines. The frequency of G:C-->C:G transversions was significantly higher with plasmids recovered from the XP variant cells than from normal cells. The location of mutations in the marker gene was non-random with different mutagenic hotspots found in plasmids recovered from the XP variant cells and from the normal cells. This study suggests that plasmid UV hypermutability in the presence of normal UV survival may be related to the increased UV skin cancer susceptibility of XP variant patients. |
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Authors:
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H L Waters; S Seetharam; M M Seidman; K H Kraemer |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: The Journal of investigative dermatology Volume: 101 ISSN: 0022-202X ISO Abbreviation: J. Invest. Dermatol. Publication Date: 1993 Nov |
Date Detail:
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Created Date: 1993-11-26 Completed Date: 1993-11-26 Revised Date: 2008-08-12 |
Medline Journal Info:
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Nlm Unique ID: 0426720 Medline TA: J Invest Dermatol Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 744-8 Citation Subset: IM |
Affiliation:
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Laboratory of Molecular Carcinogenesis, National Cancer Institute, Bethesda, Maryland 20892. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Adult Base Sequence Cell Line DNA Repair Female Genetic Vectors Humans Male Molecular Sequence Data Mutation* Plasmids Ultraviolet Rays Xeroderma Pigmentosum / genetics* |
| Grant Support | |
ID/Acronym/Agency:
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Z01 BC004517-31/BC/NCI NIH HHS |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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