Document Detail


Ultraviolet B radiation differentially modifies catechol-O-methyltransferase activity in keratinocytes and melanoma cells.
MedLine Citation:
PMID:  22548395     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
BACKGROUND: Catechol-O-methyltransferase (COMT) is a ubiquitous enzyme inactivating catecholic compounds. COMT is expressed also in human skin samples, and in melanoma cells it may be cytoprotective. A role of COMT in keratinocytes (HaCat) is unknown.
OBJECTIVE: The objective of this study is: to investigate whether ultraviolet-B (UVB) radiation modifies COMT activity in melanocytes and HaCat and whether COMT inhibition plays a role in UVB-induced cell death.
METHODS: Human cell lines of melanotic melanoma (SK-mel-1) and HaCat were used. COMT activity was evaluated under basal conditions and after UVB irradiation (311 nm) at a low (8 mJ/cm(2)) and a high dose (60 mJ/cm(2)). Tolcapone 1 μM was used to inhibit COMT.
RESULTS: Both SK-mel-1 and Ha-Cat cells express COMT activity. In SK-mel-1, COMT activity is reduced nearly 50% both 24 h and 48 h after a high dose UVB. In Ha-Cat cells, COMT activity increased 24 h after a high dose UVB but decreased at 48 h. Tolcapone increases significantly the cytotoxic effect of high dose UVB irradiation only in HaCat. High concentrations of tolcapone reduced melanin levels in melanoma cells parallel to reduced cell numbers.
CONCLUSIONS: Ultraviolet radiation differentially modifies COMT activity in melanoma cells and HaCat. Furthermore, tolcapone increased death of HaCat after irradiation but did not affect melanoma cells.
Authors:
Sofia Magina; Maria Augusta Vieira-Coelho; Maria Paula Serrão; Carina Kosmus; Eduardo Moura; Daniel Moura
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Photodermatology, photoimmunology & photomedicine     Volume:  28     ISSN:  1600-0781     ISO Abbreviation:  Photodermatol Photoimmunol Photomed     Publication Date:  2012 Jun 
Date Detail:
Created Date:  2012-05-02     Completed Date:  2012-09-04     Revised Date:  2013-11-25    
Medline Journal Info:
Nlm Unique ID:  9013641     Medline TA:  Photodermatol Photoimmunol Photomed     Country:  Denmark    
Other Details:
Languages:  eng     Pagination:  137-41     Citation Subset:  IM    
Copyright Information:
© 2012 John Wiley & Sons A/S.
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MeSH Terms
Descriptor/Qualifier:
Benzophenones / pharmacology
Catechol O-Methyltransferase / antagonists & inhibitors,  metabolism*
Cell Death / drug effects,  radiation effects
Cell Line, Tumor
Enzyme Inhibitors / pharmacology
Humans
Keratinocytes / enzymology*,  pathology
Melanoma / enzymology*,  pathology
Neoplasm Proteins / antagonists & inhibitors,  metabolism*
Nitrophenols / pharmacology
Ultraviolet Rays*
Chemical
Reg. No./Substance:
0/Benzophenones; 0/Enzyme Inhibitors; 0/Neoplasm Proteins; 0/Nitrophenols; CIF6334OLY/tolcapone; EC 2.1.1.6/Catechol O-Methyltransferase

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