| Ultrathin self-assembled fibrin sheets. | |
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MedLine Citation:
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PMID: 19052234 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Fibrin polymerizes into the fibrous network that is the major structural component of blood clots and thrombi. We demonstrate that fibrin from three different species can also spontaneously polymerize into extensive, molecularly thin, 2D sheets. Sheet assembly occurs in physiologic buffers on both hydrophobic and hydrophilic surfaces, but is routinely observed only when polymerized using very low concentrations of fibrinogen and thrombin. Sheets may have been missed in previous studies because they may be very short-lived at higher concentrations of fibrinogen and thrombin, and their thinness makes them very difficult to detect. We were able to distinguish fluorescently labeled fibrin sheets by polymerizing fibrin onto micro-patterned structured surfaces that suspended polymers 10 microm above and parallel to the cover-glass surface. We used a combined fluorescence/atomic force microscope system to determine that sheets were approximately 5 nm thick, flat, elastic and mechanically continuous. Video microscopy of assembling sheets showed that they could polymerize across 25-microm channels at hundreds of microm(2)/sec (approximately 10(13) subunits/s x M), an apparent rate constant many times greater than those of other protein polymers. Structural transitions from sheets to fibers were observed by fluorescence, transmission, and scanning electron microscopy. Sheets appeared to fold and roll up into larger fibers, and also to develop oval holes to form fiber networks that were "pre-attached" to the substrate and other fibers. We propose a model of fiber formation from sheets and compare it with current models of end-wise polymerization from protofibrils. Sheets could be an unanticipated factor in clot formation and adhesion in vivo, and are a unique material in their own right. |
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Authors:
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E Tim O'Brien; Michael R Falvo; Daniel Millard; Brian Eastwood; Russell M Taylor; Richard Superfine |
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Publication Detail:
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Type: Journal Article; Research Support, N.I.H., Extramural; Research Support, U.S. Gov't, Non-P.H.S. Date: 2008-12-03 |
Journal Detail:
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Title: Proceedings of the National Academy of Sciences of the United States of America Volume: 105 ISSN: 1091-6490 ISO Abbreviation: Proc. Natl. Acad. Sci. U.S.A. Publication Date: 2008 Dec |
Date Detail:
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Created Date: 2008-12-10 Completed Date: 2009-01-30 Revised Date: 2013-06-04 |
Medline Journal Info:
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Nlm Unique ID: 7505876 Medline TA: Proc Natl Acad Sci U S A Country: United States |
Other Details:
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Languages: eng Pagination: 19438-43 Citation Subset: IM |
Affiliation:
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Department of Physics and Astronomy, University of North Carolina, Chapel Hill, NC 27599-3255, USA. etobrien@email.unc.edu |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Blood Coagulation Chickens Fibrin / chemistry*, metabolism*, ultrastructure Fibrinogen / pharmacology Glass Humans Hydrophobic and Hydrophilic Interactions Mice Microscopy, Atomic Force Microscopy, Electron, Scanning Microscopy, Electron, Transmission Polymers / chemistry*, metabolism* Thrombin / pharmacology |
| Grant Support | |
ID/Acronym/Agency:
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P41-EB002025-23A1/EB/NIBIB NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Polymers; 9001-31-4/Fibrin; 9001-32-5/Fibrinogen; EC 3.4.21.5/Thrombin |
| Comments/Corrections | |
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