| Ultrastructure of rat atrial tissue after either perfusion-or immersion-fixation both in vivo and in vitro study: comparison of methodological reliability. | |
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MedLine Citation:
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PMID: 9651741 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The present study compares, from a morphological point of view, the possible differences between perfusion- and immersion-fixation techniques performed on rat myocardium in order to evaluate their reliability. The data we obtained showed that when the two techniques are carried out with particular care, they do not present significant differences in tissue preservation. Furthermore, we also evaluated the ultrastructural of rat myocardium at the end of in vitro experiments. In this case, signs of subcellular changes, such as vacuolar degeneration, swollen mitochondria, intermyofibrillar myelin-like figures and enlargements of non specialized junctions of the intercalated discs can frequently occur. So, our results indicate that the morphological integrity of myocardium is not completely preserved during in vitro investigations. |
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Authors:
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P Lenzi; M Gesi; F Martini; G Natale; A Pellegrini; P Soldani; A Paparelli |
Publication Detail:
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Type: Comparative Study; Journal Article |
Journal Detail:
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Title: European journal of morphology Volume: 36 ISSN: 0924-3860 ISO Abbreviation: Eur J Morphol Publication Date: 1998 Apr |
Date Detail:
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Created Date: 1998-10-09 Completed Date: 1998-10-09 Revised Date: 2007-11-15 |
Medline Journal Info:
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Nlm Unique ID: 9011462 Medline TA: Eur J Morphol Country: NETHERLANDS |
Other Details:
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Languages: eng Pagination: 77-82 Citation Subset: IM |
Affiliation:
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Institute of Normal Human Anatomy, Faculty of Medicine and Surgery, University of Pisa, Italy. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Evaluation Studies as Topic Heart Atria / ultrastructure Immersion Male Myocardium / ultrastructure* Perfusion Rats Rats, Wistar Specimen Handling / methods* Subcellular Fractions / ultrastructure Tissue Fixation / methods* |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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