Document Detail

Ultrastructural study of poly- -hydroxybutyrate granules from Bacillus cereus.
MedLine Citation:
PMID:  4123236     Owner:  NLM     Status:  MEDLINE    
The freeze-etching technique was used to examine the effects of fracturing and etching on the appearance of poly-beta-hydroxybutyrate granules from Bacillus cereus. These granules were examined in extracts isolated by hypochlorite or by sonic treatment, and also in fixed and unfixed intact cells; in the latter case they were compared with granules in thin sections of intact cells. After freeze-fracturing, the diameter of the granules in intact cells was between 240 and 720 nm. The granules consisted of a central core, of diameter between 140 and 370 nm, which occupied less than 50% of the volume of the granule and which was either stretched or removed on fracturing; the core was surrounded by an outer coat which may be bounded by a membrane.
W F Dunlop; A W Robards
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Journal of bacteriology     Volume:  114     ISSN:  0021-9193     ISO Abbreviation:  J. Bacteriol.     Publication Date:  1973 Jun 
Date Detail:
Created Date:  1973-08-21     Completed Date:  1973-08-21     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  2985120R     Medline TA:  J Bacteriol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  1271-80     Citation Subset:  IM    
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MeSH Terms
Bacillus cereus / cytology*
Culture Media
Freeze Etching
Hydroxybutyrates / analysis*
Microscopy, Electron
Staining and Labeling
Time Factors
Reg. No./Substance:
0/Culture Media; 0/Hydroxybutyrates; 0/Polymers

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