Document Detail


Ultrastructural proof of polyomavirus in Merkel cell carcinoma tumour cells and its absence in small cell carcinoma of the lung.
MedLine Citation:
PMID:  19305499     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
BACKGROUND: A new virus called the Merkel Cell Polyomavirus (MCPyV) has recently been found in Merkel Cell Carcinoma (MCC). MCC is a rare aggressive small cell neuroendocrine carcinoma primarily derived from the skin, morphologically indistinguishable from small cell lung carcinoma (SCLC). So far the actual presence of the virus in MCC tumour cells on a morphological level has not been demonstrated, and the presence of MCPyV in other small cell neuroendocrine carcinomas has not been studied yet.
METHODOLOGY/PRINCIPAL FINDINGS: We investigated MCC tissue samples from five patients and SCLCs from ten patients for the presence of MCPyV-DNA by PCR and sequencing. Electron microscopy was used to search ultrastructurally for morphological presence of the virus in MCPyV-DNA positive samples. MCPyV was detected in two out of five primary MCCs. In one MCC patient MCPyV-DNA was detected in the primary tumour as well as in the metastasis, strongly suggesting integration of MCPyV in the cellular DNA of the tumour in this patient. In the primary MCC of another patient viral particles in tumour cell nuclei and cytoplasm were identified by electron microscopy, indicating active viral replication in the tumour cells. In none of the SCLCs MCPyV-DNA was detected.
CONCLUSIONS/SIGNIFICANCE: Our results strongly suggest that MCPyV is an oncogenic polyomavirus in humans, and is potentially causally related to the development of MCC but not to the morphological similar SCLC.
Authors:
Charlotte T A H Wetzels; Jolanda G M Hoefnagel; Judith M J E Bakkers; Henry B P M Dijkman; Willeke A M Blokx; Willem J G Melchers
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Publication Detail:
Type:  Journal Article     Date:  2009-03-23
Journal Detail:
Title:  PloS one     Volume:  4     ISSN:  1932-6203     ISO Abbreviation:  PLoS ONE     Publication Date:  2009  
Date Detail:
Created Date:  2009-03-23     Completed Date:  2009-04-24     Revised Date:  2013-06-02    
Medline Journal Info:
Nlm Unique ID:  101285081     Medline TA:  PLoS One     Country:  United States    
Other Details:
Languages:  eng     Pagination:  e4958     Citation Subset:  IM    
Affiliation:
Department of Pathology, Radboud University Nijmegen Medical Centre, Nijmegen, the Netherlands. C.Wetzels@pathol.umcn.nl
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MeSH Terms
Descriptor/Qualifier:
Adult
Aged
Carcinoma, Merkel Cell* / pathology,  virology
Female
Humans
Lung Neoplasms* / pathology,  virology
Male
Middle Aged
Polyomavirus / genetics,  ultrastructure*
Polyomavirus Infections / genetics,  pathology,  virology
Skin Neoplasms* / pathology,  virology
Small Cell Lung Carcinoma* / pathology,  virology
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