| Ultrastructural morphometric analysis of the uterine epithelium during early pregnancy in the sheep. | |
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MedLine Citation:
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PMID: 2401979 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Stereological techniques were used to quantify ultrastructural changes in the caruncular epithelium during the pre- (Day 13), peri- (Day 16) and post- (Days 19 and 22) attachment periods of placentation. Tissues from Day-13 non-pregnant ewes were used as controls. Uteri for stereological evaluation were perfused via the uterine artery with 3% glutaraldehyde and separated into proximal, middle and distal regions. Tissues from caruncular areas were processed for electron microscopy. Volume fractions (Vv) of nuclei, mitochondria, lipid and cytoplasmic granules were estimated by point-counting volumetry. Surface areas per unit tissue volume (Sv) of mitochondrial membranes and cristae, Golgi, plasmalemma, endoplasmic reticulum and nuclear membranes were estimated by line-intersection counting. The only significant difference between pregnant and non-pregnant uterine epithelium at Day 13, a time before attachment, was a lower Sv of smooth endoplasmic reticulum (SER) in tissue from pregnant ewes. This value returned to control (non-pregnant Day 13) levels at Day 16, and was again significantly reduced at Days 19 and 22. The Vv of lipid decreased significantly at Day 16 and remained at reduced levels thereafter. These changes may reflect the effects of conceptus products on lipid storage and mobilization. The Sv of rough endoplasmic reticulum (RER) significantly increased on Day 16 of gestation, and remained elevated on Day 19. These results may reflect increased synthesis of protein for export at these times. In general, several of the values measured which may be indicative of cellular metabolism were reduced at Day 22 of pregnancy, perhaps suggesting diminished metabolism by the uterine epithelium after attachment of the trophoblast.(ABSTRACT TRUNCATED AT 250 WORDS) |
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Authors:
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S E Smith; W C Cullen; J D Godkin |
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Publication Detail:
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Type: Journal Article; Research Support, U.S. Gov't, Non-P.H.S. |
Journal Detail:
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Title: Journal of reproduction and fertility Volume: 89 ISSN: 0022-4251 ISO Abbreviation: J. Reprod. Fertil. Publication Date: 1990 Jul |
Date Detail:
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Created Date: 1990-10-24 Completed Date: 1990-10-24 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 0376367 Medline TA: J Reprod Fertil Country: ENGLAND |
Other Details:
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Languages: eng Pagination: 517-25 Citation Subset: IM |
Affiliation:
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Department of Animal Science, University of Tennessee, Knoxville 37901. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Epithelium / ultrastructure Female Microscopy, Electron Placentation / physiology Pregnancy Pregnancy, Animal / physiology* Sheep / anatomy & histology*, physiology Uterus / ultrastructure* |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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