Document Detail

Ultrastructural and molecular changes in the left and right ventricular myocardium associated with ascites syndrome in broiler chickens raised at low altitude.
MedLine Citation:
PMID:  11515307     Owner:  NLM     Status:  MEDLINE    
The present study examines ultrastructural and molecular changes in ventricular myocardium associated with ascites cases in fast-growing broilers raised at low altitude. Extensive ultrastructural lesions were seen in the left and right ventricular myocardium of broilers with fulminant heart failure and ascites. Significant changes included lesions in the myofibril contractile apparatus, altered mitochondria, marked reduction in the myofibril component, and changes in the extracellular matrix (ECM) architecture. No lesions were observed in hearts of slow growing broilers, but mild to moderate changes (predominantly in the left ventriculum) were apparent in the hearts from some clinically normal, fast-growing broilers. SDS-PAGE profiles of washed myofibrils showed several distinctly different bands in preparations from left ventricular myocardium of ascitic birds. Western blot analysis of these samples revealed several fragments of myosin heavy chain, M-protein, and titin. Based on gelatinolytic activity, matrix metalloproteinases (MMP) in the cytosolic fraction of ventricular myocardium homogenates were identified as MMP-2. The relative activity of this enzyme appears to be considerably higher in preparations from broilers, particularly in the preparations from the left ventriculum of fast-growing broilers, in comparison to leghorns or slow growing broilers. The nature and distribution of the changes in the heart indicate that chronic cardiomyopathic process in the left ventricular myocardium occurs during the development of ascites. It is postulated that progressive deterioration of the left heart pump function caused by initial lesions in the left ventricular myocardium is a significant factor in the development of pulmonary hypertension and the pathogenesis of ascites in broilers raised at low altitude.
A A Olkowski; B M Rathgeber; G Sawicki; H L Classen
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Journal of veterinary medicine. A, Physiology, pathology, clinical medicine     Volume:  48     ISSN:  0931-184X     ISO Abbreviation:  J Vet Med A Physiol Pathol Clin Med     Publication Date:  2001 Feb 
Date Detail:
Created Date:  2001-08-22     Completed Date:  2001-10-04     Revised Date:  2003-11-14    
Medline Journal Info:
Nlm Unique ID:  100955112     Medline TA:  J Vet Med A Physiol Pathol Clin Med     Country:  Germany    
Other Details:
Languages:  eng     Pagination:  1-14     Citation Subset:  IM    
Department of Animal and Poultry Science, University of Saskatchewan, 72 Campus Drive, Saskatoon, SK, S7N 5B5, Canada.
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MeSH Terms
Ascites / pathology,  veterinary*
Electrophoresis, Polyacrylamide Gel / veterinary
Heart Ventricles / pathology
Hypertension, Pulmonary / pathology,  veterinary
Microscopy, Electron, Scanning / veterinary
Myocardium / ultrastructure*
Poultry Diseases / pathology*

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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