| Ultrastructural localization of nucleic acids through several cytochemical techniques on osmium-fixed tissues: comparative evaluation of the different labelings. | |
| | |
MedLine Citation:
|
PMID: 6208238 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
Several cytochemical techniques, such as sodium tungstate, acid hydrolysis phosphotungstic acid (HAPTA), ethylenediaminetetraacetic acid (EDTA), RNase-gold, and osmium-ammine, have been applied for the ultrastructural demonstration of nucleic acids on sections of tissues fixed in glutaraldehyde postfixed with osmium tetroxide and embedded in Epon. In order to obtain specific results, the sections had to be treated with sodium metaperiodate prior to performing the labeling protocol. The results for each method were identical to those obtained on nonosmicated tissues; the main difference being the enhancement in the ultrastructural preservation, which allowed for higher resolution. In addition to these techniques, and for comparative evaluations, DNA was also revealed by the DNase-gold approach on nonosmicated tissue sections. The consistency in the results, obtained over the nucleus with either EDTA or the RNase-gold complex for revealing RNA and those obtained with either osmium-ammine or DNase-gold for revealing DNA, supports the high specificity of the RNase-gold, DNase-gold, and osmium-ammine techniques. Furthermore, these results demonstrate the possibility of performing various cytochemical techniques on tissues processed for routine electron microscopy. |
| | |
Authors:
|
M Bendayan; E Puvion |
Related Documents
:
|
8344408 - Gametocytogenesis induction by berenil in cultured plasmodium falciparum. 461188 - Neighbouring group participation in the unblocking of phosphotriesters of nucleic acids. 20135118 - Peptide nucleic acids (pnas) antisense effect to bacterial growth and their application... 18968108 - Ftir spectroscopy studies of nucleic acid damage. 18963318 - Alizarin-9-imine as indicator in acetonitrile and in pyridine media. 15921428 - Macrophage activating effects of new alkamides from the roots of echinacea species. |
Publication Detail:
|
Type: Comparative Study; Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
|
Title: The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society Volume: 32 ISSN: 0022-1554 ISO Abbreviation: J. Histochem. Cytochem. Publication Date: 1984 Nov |
Date Detail:
|
Created Date: 1984-12-05 Completed Date: 1984-12-05 Revised Date: 2006-11-15 |
Medline Journal Info:
|
Nlm Unique ID: 9815334 Medline TA: J Histochem Cytochem Country: UNITED STATES |
Other Details:
|
Languages: eng Pagination: 1185-91 Citation Subset: IM |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Animals DNA / metabolism* Fixatives / diagnostic use* Histocytochemistry / methods* Image Enhancement / methods Liver / metabolism Microscopy, Electron Osmium / diagnostic use* Pancreas / metabolism RNA / metabolism* Rats |
| Chemical | |
Reg. No./Substance:
|
0/Fixatives; 63231-63-0/RNA; 7440-04-2/Osmium; 9007-49-2/DNA |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Light and electron microscopic demonstration of sialic acid residues with the lectin from Limax flav...
Next Document: Arginine-rich proteins in spherical inclusions of human locus coeruleus neurons demonstrated by benz...