Document Detail


Ultrastructural localization of lectin-binding sites in normal skin.
MedLine Citation:
PMID:  2313119     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The distribution of carbohydrate residues in keratinocytes of normal epidermis was studied. Normal skin was embedded in Lowicryl. Thin sections were incubated with concanavalin A (Con A), peanut agglutinin (PNA), wheat germ agglutinin (WGA), Ulex europaeus agglutinin I (UEA I), dolichos biflorus agglutinin (DBA), and soybean agglutinin (SBA). A positive reaction in the dermis, in the basal lamina (lamina densa, lamina lucida), intracellularly and within the plasma membrane including the desmosomes was obtained after incubation with Con A and WGA. PNA binding sites were found predominantly in the plasma membrane between the desmosomes. The labeling with Con A, WGA, and PNA was most pronounced in the upper stratum spinosum and granulosum. Incubation with UEA revealed heavy labeling of the keratohyalin granules and the cytoplasm of the corneocytes. Incubation with DBA and SBA revealed weak labeling of the keratinocytes. The study of the distribution of carbohydrate residues in normal epidermis is important, since alterations in this distribution might be linked to autoimmunity or malignant transformation.
Authors:
G Schaumburg-Lever
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  The Journal of investigative dermatology     Volume:  94     ISSN:  0022-202X     ISO Abbreviation:  J. Invest. Dermatol.     Publication Date:  1990 Apr 
Date Detail:
Created Date:  1990-04-23     Completed Date:  1990-04-23     Revised Date:  2005-11-17    
Medline Journal Info:
Nlm Unique ID:  0426720     Medline TA:  J Invest Dermatol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  465-70     Citation Subset:  IM    
Affiliation:
Department of Dermatology, Eberhard-Karls-Universität, Tübingen, West Germany.
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MeSH Terms
Descriptor/Qualifier:
Carbohydrate Metabolism
Concanavalin A / metabolism
Humans
Lectins / metabolism
Peanut Agglutinin
Plant Lectins*
Receptors, Mitogen / analysis*
Skin / ultrastructure*
Soybean Proteins*
Wheat Germ Agglutinins / metabolism
Chemical
Reg. No./Substance:
0/Lectins; 0/Peanut Agglutinin; 0/Plant Lectins; 0/Receptors, Mitogen; 0/Soybean Proteins; 0/Ulex europaeus lectins; 0/Wheat Germ Agglutinins; 0/dolichos biflorus agglutinin; 0/soybean lectin; 11028-71-0/Concanavalin A

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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