| Ultrastructural localization of gamma amino butyric acid immunoreactivity in B cells of the rat pancreas. | |
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MedLine Citation:
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PMID: 3552825 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The purpose of this study was to determine the ultrastructural localization of gamma amino butyric acid (GABA) within the B cell of isolated rat islets, particularly with respect to the B cell secretion granules. GABA immunoreactivity was localized ultrastructurally, with colloidal gold, in the B cells and absent in the A cells and D cells. Quantitative analysis of the colloidal gold particle distribution in the B cell revealed 29.5 +/- 5.2 gold particles/micron 2 in the nuclei, 29.3 +/- -6.9 gold particles/micron 2 in the mitochondria and 4.9 +/- 1.0 gold particles/micron 2 in the secretion granules. Particle density in the remainder of the cytoplasm was 41.9 +/- 4.1 particles/micron 2. The paucity of gamma amino butyric acid in the B cell secretion granules differs from observations on gamma amino butyric acidergic neurons, where there is an accumulation of gamma amino butyric acid within the neurosecretory vesicles. These findings indicate that if gamma amino butyric acid is released from the islet, then it is by a nongranular mechanism. In addition, the results are compatible with the hypotheses that gamma amino butyric acid within the B cell functions in the regulation of insulin biosynthesis, and/or functions as an alternative energy source for the B cell through the gamma amino butyric acid shunt. |
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Authors:
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D J Garry; R L Sorenson; H D Coulter |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: Diabetologia Volume: 30 ISSN: 0012-186X ISO Abbreviation: Diabetologia Publication Date: 1987 Feb |
Date Detail:
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Created Date: 1987-06-09 Completed Date: 1987-06-09 Revised Date: 2007-11-14 |
Medline Journal Info:
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Nlm Unique ID: 0006777 Medline TA: Diabetologia Country: GERMANY, WEST |
Other Details:
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Languages: eng Pagination: 115-9 Citation Subset: IM |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Histocytochemistry Immunochemistry Insulin / analysis, biosynthesis Islets of Langerhans / analysis*, ultrastructure Male Rats Rats, Inbred Strains gamma-Aminobutyric Acid / analysis, immunology* |
| Grant Support | |
ID/Acronym/Agency:
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AM 33655/AM/NIADDK NIH HHS |
| Chemical | |
Reg. No./Substance:
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11061-68-0/Insulin; 56-12-2/gamma-Aminobutyric Acid |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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