| Ultrastructural localization of albumin transport across the cerebral microvasculature during experimental meningitis in the rat. | |
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MedLine Citation:
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PMID: 1875166 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Injury to the blood brain barrier (BBB) is a fundamental sequela of bacterial meningitis, yet the precise mechanism facilitating exudation of albumin across the endothelium of the cerebral microvasculature remains conjectural. After intracisternal inoculation of Escherichia coli (0111:B4) lipopolysaccharide in rats to elicit a reversible meningitis and BBB injury, we utilized in situ tracer perfusion and immunolabeling procedures to identify by transmission electron microscopy the precise topography and microvascular exit pathway(s) of bovine serum albumin (BSA). Results revealed that during meningitis there was: (a) an inducible increase in immunodetectable monomeric BSA binding to the luminal membrane of all microvascular segments in the pia-arachnoid and superficial brain cortex; (b) similar uptake of both colloidal Au-BSA (as well as monomeric BSA) by plasmalemmal vesicles but no detectable transcytosis to the abluminal side; and (c) predominant exit of both perfused Au-BSA and immunodetectable monomeric BSA through open intercellular junctions of venules in the pia-arachnoid. This was corroborated in separate experiments documenting focal pial venular leaks of in situ perfused 0.01% colloidal carbon black during experimental meningitis. These results provide precise localization of BBB injury in meningitis to meningeal venules, confirm a paracellular exit pathway of albumin via open intercellular junctions, and suggest an injury mechanism amenable to specific therapeutic intervention. |
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Authors:
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V J Quagliarello; A Ma; H Stukenbrok; G E Palade |
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Publication Detail:
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Type: Journal Article; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: The Journal of experimental medicine Volume: 174 ISSN: 0022-1007 ISO Abbreviation: J. Exp. Med. Publication Date: 1991 Sep |
Date Detail:
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Created Date: 1991-09-26 Completed Date: 1991-09-26 Revised Date: 2009-11-18 |
Medline Journal Info:
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Nlm Unique ID: 2985109R Medline TA: J Exp Med Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 657-72 Citation Subset: IM |
Affiliation:
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Department of Internal Medicine, Yale University School of Medicine, New Haven, Connecticut 06510. |
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Biological Transport Blood-Brain Barrier* Carbon Escherichia coli Immunohistochemistry Lipopolysaccharides / administration & dosage Male Meningitis / metabolism*, pathology Microcirculation / metabolism* Rats Rats, Inbred Strains Serum Albumin, Bovine / metabolism* |
| Grant Support | |
ID/Acronym/Agency:
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5-K11-HL02062-03/HL/NHLBI NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Lipopolysaccharides; 0/Serum Albumin, Bovine; 7440-44-0/Carbon |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
| Full Text | |
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Journal Information Journal ID (nlm-ta): J Exp Med ISSN: 0022-1007 ISSN: 1540-9538 Publisher: The Rockefeller University Press |
Article Information Download PDF  Print publication date: Day: 1 Month: 9 Year: 1991 Volume: 174 Issue: 3 First Page: 657 Last Page: 672 ID: 2118932 Publisher Id: 91341426 PubMed Id: 1875166 |
| Ultrastructural localization of albumin transport across the cerebral microvasculature during experimental meningitis in the rat | |
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