Document Detail

Ultrastructural and enzymic modulation of HeLa cells induced by sodium butyrate and the effects of cytochalasin B and colcemid.
MedLine Citation:
PMID:  972176     Owner:  NLM     Status:  MEDLINE    
Sodium butyrate causes HeLa cells to assume an elongated and jagged shape. Ultrastructurally this change is associated with the formation of bundles of microfilaments. Desmosomes were present between adjacent cells. No increase in microtubules was observed in the butyrate-treated cells. Butyrate induces an increase in the activity of 2 membrane-bound enzymes, alkaline phosphatase and 5'-nucleotidase; however, the activity of a third membrane enzyme, acetylcholine esterase, is reduced. The activities of the several other enzymes with different subcellular localizations are not significantly increased. Colcemid and cytochalasin B prevent or reverse the butyrate-mediated change in HeLa cell morphology and also partially inhibit the induction of alkaline phosphatase activity in these cells. The effect of cytochalasin B on alkaline phosphatase induction may be caused by a reduction in protein synthesis produced by this fungal metabolite.
S I Deutsch; D N Silvers; R P Cox; M J Griffin; N K Ghosh
Related Documents :
22489176 - Cellulose biosynthesis inhibitors: comparative effect on bean cell cultures.
19333286 - Trap profiles of projector based optoelectronic tweezers (oet) with hela cells.
2869966 - Immunochemical and biochemical analysis of the proliferating cell nuclear antigen (pcna...
20684966 - Inhibition of nuclear entry of hpv16 pseudovirus-packaged dna by an anti-hpv16 l2 neutr...
22151756 - Overexpression of id3 induces apoptosis of a549 human lung adenocarcinoma cells.
8259526 - Inhibition of a9 and a10 dopamine cells by the cholecystokinin-b antagonist ly262691: m...
Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Journal of cell science     Volume:  21     ISSN:  0021-9533     ISO Abbreviation:  J. Cell. Sci.     Publication Date:  1976 Jul 
Date Detail:
Created Date:  1976-12-03     Completed Date:  1976-12-03     Revised Date:  2009-11-03    
Medline Journal Info:
Nlm Unique ID:  0052457     Medline TA:  J Cell Sci     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  391-406     Citation Subset:  IM    
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Acid Phosphatase / metabolism
Alkaline Phosphatase / metabolism
Butyrates / pharmacology*
Cell Membrane / enzymology,  ultrastructure
Cholinesterases / metabolism
Colchicine / pharmacology*
Cytochalasin B / pharmacology*
Desmosomes / ultrastructure
Esterases / metabolism*
Hela Cells / ultrastructure*
Neoplasm Proteins / biosynthesis
Nucleotidases / metabolism
Reg. No./Substance:
0/Butyrates; 0/Neoplasm Proteins; 14930-96-2/Cytochalasin B; 64-86-8/Colchicine; EC 3.1.-/Esterases; EC; EC 3.1.3.-/Nucleotidases; EC Phosphatase; EC Phosphatase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  The blepharoplast of Marsilea: its de novo formation and spindle association.
Next Document:  Pollen-wall proteins: quantitative cytochemistry of the origins of intine and exine enzymes in Brass...