Document Detail


Ultrastructural and enzymic modulation of HeLa cells induced by sodium butyrate and the effects of cytochalasin B and colcemid.
MedLine Citation:
PMID:  972176     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Sodium butyrate causes HeLa cells to assume an elongated and jagged shape. Ultrastructurally this change is associated with the formation of bundles of microfilaments. Desmosomes were present between adjacent cells. No increase in microtubules was observed in the butyrate-treated cells. Butyrate induces an increase in the activity of 2 membrane-bound enzymes, alkaline phosphatase and 5'-nucleotidase; however, the activity of a third membrane enzyme, acetylcholine esterase, is reduced. The activities of the several other enzymes with different subcellular localizations are not significantly increased. Colcemid and cytochalasin B prevent or reverse the butyrate-mediated change in HeLa cell morphology and also partially inhibit the induction of alkaline phosphatase activity in these cells. The effect of cytochalasin B on alkaline phosphatase induction may be caused by a reduction in protein synthesis produced by this fungal metabolite.
Authors:
S I Deutsch; D N Silvers; R P Cox; M J Griffin; N K Ghosh
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Journal of cell science     Volume:  21     ISSN:  0021-9533     ISO Abbreviation:  J. Cell. Sci.     Publication Date:  1976 Jul 
Date Detail:
Created Date:  1976-12-03     Completed Date:  1976-12-03     Revised Date:  2009-11-03    
Medline Journal Info:
Nlm Unique ID:  0052457     Medline TA:  J Cell Sci     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  391-406     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Acid Phosphatase / metabolism
Alkaline Phosphatase / metabolism
Butyrates / pharmacology*
Cell Membrane / enzymology,  ultrastructure
Cholinesterases / metabolism
Colchicine / pharmacology*
Cytochalasin B / pharmacology*
Desmosomes / ultrastructure
Esterases / metabolism*
Hela Cells / ultrastructure*
Neoplasm Proteins / biosynthesis
Nucleotidases / metabolism
Chemical
Reg. No./Substance:
0/Butyrates; 0/Neoplasm Proteins; 14930-96-2/Cytochalasin B; 64-86-8/Colchicine; EC 3.1.-/Esterases; EC 3.1.1.8/Cholinesterases; EC 3.1.3.-/Nucleotidases; EC 3.1.3.1/Alkaline Phosphatase; EC 3.1.3.2/Acid Phosphatase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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