| Ultrastructural distribution of cell surface antigens in avian tumor virus-infected chick embryo fibroblasts. | |
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MedLine Citation:
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PMID: 4134462 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The distribution of neoantigens in the surface membrane of avian tumor virus-infected chicken embryo fibroblasts was examined on carbon replicas of cell cultures using hemocyanin-labeled antibody. New determinants appearing on the cell surface of virally infected but not transformed cells are thought to be common with components of the viral envelope. These antigens were found to exist in a diffuse, random array on the dorsal cell surface, with a denser accumulation along the cell processes. In living cells, surface antigens are capable of several types of redistribution when activated by reaction with antibody. Leukosis virus-infected (non-transformed) cells showed two apparently independent modes of redistribution: a relocation of some antibody-related sites to the cell margin; or an involvement of essentially all sites in randomly dispersed aggregates. Viral antigenic sites on sarcoma virus-infected (transformed) cells, reacted with antibody, were able to produce weak marginal relocation; but revealed a more striking tendency to migrate to some central location. The centripetal coalescence thus formed resembles the "cap" noted in other systems. Prior aggregation into "patches" may not be a prerequisite for such cap formation. Tumor-specific surface antigen detection and mapping was attempted by this technique, but results were equivocal. An antigen possibly characteristic of rapidly dividing cells occurred in a sparse, diffuse fashion over the surface of morphologically distinct "round" cells. |
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Authors:
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E R Phillips; J F Perdue |
Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: The Journal of cell biology Volume: 61 ISSN: 0021-9525 ISO Abbreviation: J. Cell Biol. Publication Date: 1974 Jun |
Date Detail:
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Created Date: 1974-08-26 Completed Date: 1974-08-26 Revised Date: 2010-06-22 |
Medline Journal Info:
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Nlm Unique ID: 0375356 Medline TA: J Cell Biol Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 743-56 Citation Subset: IM |
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Antibody Specificity Antigens, Neoplasm* Antigens, Viral* Avian Sarcoma Viruses / immunology* Avian leukosis virus / immunology* Binding Sites, Antibody Cell Membrane / immunology* Cell Transformation, Neoplastic Cells, Cultured Chick Embryo Epitopes Fibroblasts / immunology Hemocyanin Immune Sera Immunoglobulin G Microscopy, Electron Rabbits / immunology |
| Chemical | |
Reg. No./Substance:
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0/Antigens, Neoplasm; 0/Antigens, Viral; 0/Binding Sites, Antibody; 0/Epitopes; 0/Immune Sera; 0/Immunoglobulin G; 9013-72-3/Hemocyanin |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
| Full Text | |
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Journal Information Journal ID (nlm-ta): J Cell Biol ISSN: 0021-9525 ISSN: 1540-8140 Publisher: The Rockefeller University Press |
Article Information Download PDF  Copyright © 1974 by The Rockefeller University Press Received Day: 12 Month: 11 Year: 1973 Revision Received Day: 14 Month: 1 Year: 1974 Print publication date: Day: 1 Month: 6 Year: 1974 Volume: 61 Issue: 3 First Page: 743 Last Page: 756 ID: 2109314 PubMed Id: 4134462 |
| ULTRASTRUCTURAL DISTRIBUTION OF CELL SURFACE ANTIGENS IN AVIAN TUMOR VIRUS-INFECTED CHICK EMBRYO FIBROBLASTS | |
| E. R. Phillips | |
| J. F. Perdue | |
| From the McArdle Laboratory for Cancer Research, University of Wisconsin Medical School, Madison, Wisconsin 53706. |
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| Dr. Phillips's and Dr. Perdue's present address is the Lady Davis Institute for Medical Research of the National Jewish, Hospitals, Montreal, Quebec, Canada. |
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