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Ultrastructural cytochemistry. Enzyme and acid hydrolysis of nucleic acids and protein.
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MedLine Citation:
PMID:  13760208     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Selective extraction of specific cell components by enzyme or acid hydrolysis is possible from ultrathin sections for electron microscopy and parallel 2 micro sections for light microscopy of tissues fixed in formalin and embedded in a water-soluble polyepoxide, product X133/2097. Normal rat tissues fixed 15 minutes in formalin at 3 degrees C are more rapidly digested by proteinases than those fixed for the same length of time at 20 degrees C. Trypsin selectively attacks the nuclear chromatin and the ribonucleoprotein particles of the ergastroplasm, whereas mitochondria and zymogen granules resist tryptic digestion. Pepsin rapidly attacks the mitochondria and zymogen granules. The ergastoplasm and nucleus at first resist peptic digestion, but in time the entire cytoplasm and interchromatinic portion of the nucleus are attacked. Ribonuclease abolishes cytoplasmic basophilia in 2 micro sections, but parallel ultra-thin sections, stained with uranyl acetate and examined in the electron microscope, show no change in the ribonucleoprotein particles of the ergastoplasm. Desoxyribonuclease alone had no effect, but after pretreatment of the sections with pepsin or hydrochloric acid, desoxyribonuclease specifically attacked the nuclear chromatin. Nucleic acid-containing structures in the sections are gradually disintegrated by perchloric acid or hydrochloric acid.
Authors:
E H LEDUC; W BERNHARD
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  The Journal of biophysical and biochemical cytology     Volume:  10     ISSN:  0095-9901     ISO Abbreviation:  J Biophys Biochem Cytol     Publication Date:  1961 Jul 
Date Detail:
Created Date:  1961-12-01     Completed Date:  1998-11-01     Revised Date:  2010-06-22    
Medline Journal Info:
Nlm Unique ID:  17840020R     Medline TA:  J Biophys Biochem Cytol     Country:  Not Available    
Other Details:
Languages:  eng     Pagination:  437-55     Citation Subset:  OM    
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MeSH Terms
Descriptor/Qualifier:
Cell Biology*
Nucleic Acids / chemistry*
Proteins / chemistry*
Chemical
Reg. No./Substance:
0/Nucleic Acids; 0/Proteins
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Full Text
Journal Information
Journal ID (nlm-ta): J Biophys Biochem Cytol
ISSN: 0095-9901
Publisher: The Rockefeller University Press
Article Information
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Copyright © Copyright, 1961, by The Rockefeller Institute Press
Received Day: 27 Month: 1 Year: 1961
Print publication date: Day: 1 Month: 7 Year: 1961
Volume: 10 Issue: 3
First Page: 437 Last Page: 455
ID: 2225089
PubMed Id: 13760208

ULTRASTRUCTURAL CYTOCHEMISTRY : Enzyme and Acid Hydrolysis of Nucleic Acids and Protein
Elizabeth H. Leduc
Wilhelm Bernhard
From the Institute of Cancer Research, Villejuif, France.
Dr. Leduc's present address is Department of Biology, Brown University, Providence


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