Document Detail


Ultrastructural and cytochemical characterization of follicular cell types in bovine (Bos taurus) cumulus-oocyte complexes aspirated from small and medium antral follicles during the estrus cycle.
MedLine Citation:
PMID:  21167664     Owner:  NLM     Status:  In-Data-Review    
Abstract/OtherAbstract:
Stereological quantitative methods have revealed the presence of three distinct follicular cell populations (C1-C3) in bovine cumulus-oocyte complexes. Type C3 cells became largely predominant from metestrous to proestrous, with a simultaneously large decrease in the other two cell types. To further characterize these cumulus cell types, cumulus-oocyte complexes from small (1-4mm) and medium (4-8mm) antral follicles (category 1: with a compact multilayered cumulus and a homogeneous ooplasm) were aspirated from ovaries of Holstein-Friesian cows and processed for electron microscopy, ultrastructural cytochemical detection of glycogen and glycoproteins, and immunogold localization of chondroitin sulfate. Each follicular cell type displayed the same ultrastructural characteristics independently of the size of the follicle and the stage of the estrous cycle. Type C1 cells showed morphological characteristics of undifferentiated cells and progressively transformed into type C2 cells. Type C2 cells were characterized by cell extensions, polarized nuclei with evident nucleolar fibrilar centers, steroidogenic characteristics (numerous large lipid droplets, large endoplasmic reticulum vesicles and vacuoles), and synthesis of glycoproteins and chondroitin sulfate by the Golgi apparatus and endoplasmic reticulum. Type C3 cells presented morphologic features of fully differentiated and luteinized cumulus cells. They were characterized by an increase in cytoskeleton filaments, loss of cell extensions and of intercellular junctions, depletion of lipid and glycogen stores, and initiation of glycoprotein and chondroitin sulfate exocytosis. In conclusion, the present study suggests that bovine cumulus cells from small and medium antral follicles follow a complete dynamic functional differentiation process, in which the three cell types seem to correspond to a functional continuum. We identified undifferentiated cells, cells strongly engaged in glycoprotein and proteoglycan synthesis, and cells fully differentiated and secreting.
Authors:
Ana M Calado; Elsa Oliveira; Aura Colaço; Mário Sousa
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Publication Detail:
Type:  Journal Article     Date:  2010-11-30
Journal Detail:
Title:  Animal reproduction science     Volume:  123     ISSN:  1873-2232     ISO Abbreviation:  Anim. Reprod. Sci.     Publication Date:  2011 Jan 
Date Detail:
Created Date:  2011-01-25     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  7807205     Medline TA:  Anim Reprod Sci     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  23-31     Citation Subset:  IM    
Copyright Information:
Copyright © 2010 Elsevier B.V. All rights reserved.
Affiliation:
Department of Veterinary Sciences, University of Trás-os-Montes and Alto-Douro, Apartado 1013, Vila Real, Portugal.
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