Document Detail

Ultrastructural characterization of the new NG97ht human-derived glioma cell line using two different electron microscopy technical procedures.
MedLine Citation:
PMID:  19009596     Owner:  NLM     Status:  MEDLINE    
On the basis of transmission electron microscopy observations in tumor cell lines, oncologists have made innumerous diagnostic and therapeutical progresses. Following this path, the UNICAMP immunopathologies laboratory established the NG97 cell line derived from a human astrocytoma grade III, which when injected to the athymic nude mouse flank developed a grade IV astrocytoma. In this study, we focused on ultrastructural characterization of the NG97 cells after being recovered from xenotransplant (NG97ht). These cells in culture were assayed by two different electron microscopy procedures to characterize ultrastructures related to grade IV astrocytomas and to observe their structures through cell subcultivation. Additionally, comparative morphological descriptions of different cell passages in these technical procedures could be a useful tool for improving electron microscopy cell lineage protocols. Results from many cell passage observations showed ultrastructural similarities, which suggest malignant and glioblastoma phenotypes. In the first procedure, NG97ht cells were harvested and then incorporated into agarose before subjecting them to electron microscopy protocols, whereas in the second one, monolayer cells grew first on cover slides. Comparison among protocols revealed that organelles, cytoplasmatic extensions, spatial conformation of filopodia, and cell attachment to substrate were more preserved in the second procedure. Furthermore, in this latter procedure, a unique ellipsoidal structure was observed, which was already described when dealing with gliosarcoma cell line elsewhere. Therefore, these analyses demonstrated a morphological characterization of a new NG97ht cell line using electron transmission microscopy. Moreover, it has been shown that the second procedure provides more detailed information compared with the first.
Camila Maria Longo Machado; Tatiane Queiroz Zorzeto; Juares E Romero Bianco; Renata Giardini Rosa; Selma Candelaria Genari; Paulo Pinto Joazeiro; Liana Verinaud
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Publication Detail:
Type:  Evaluation Studies; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Microscopy research and technique     Volume:  72     ISSN:  1097-0029     ISO Abbreviation:  Microsc. Res. Tech.     Publication Date:  2009 Apr 
Date Detail:
Created Date:  2009-03-25     Completed Date:  2009-05-05     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9203012     Medline TA:  Microsc Res Tech     Country:  United States    
Other Details:
Languages:  eng     Pagination:  310-6     Citation Subset:  IM    
Department of Microbiology and Immunology, Biology Institute, State University of Campinas (UNICAMP), Campinas, São Paulo, Brazil.
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MeSH Terms
Astrocytoma / pathology,  ultrastructure*
Cell Culture Techniques / methods
Cell Line, Tumor
Microscopy, Electron, Transmission / methods*
Neoplasm Transplantation
Transplantation, Heterologous

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