| Ultrastructural changes in human skin mast cells during antigen-induced degranulation in vivo. | |
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MedLine Citation:
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PMID: 3204257 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Human skin mast cells were degranulated in vivo by intradermal injection of antigen. Biopsy specimens of control and stimulated skin were obtained at 5 minutes, again at 10 to 15 minutes, and analyzed by electron microscopy. Degranulation, defined as dissolution of granule contents and swelling in at least 20% of secretory granules of mast cells was observed in one third of mast cells at 5 minutes and in nearly two thirds of mast cells at 10 to 15 minutes, but was absent in biopsy specimens of control subjects. Anaphylactic degranulation was characterized by extensive fusion of granules to form degranulation channels, fusion of channels with the cell membrane to form large pores, and exocytosis of amorphous or fibrillar granule matrix into the connective tissue. Extruded secretory granules of skin mast cells persist for at least 15 minutes in the connective tissues, in apparent contrast to the process of in vivo degranulation in nasal and lung mast cells. |
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Authors:
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M M Friedman; M Kaliner |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: The Journal of allergy and clinical immunology Volume: 82 ISSN: 0091-6749 ISO Abbreviation: J. Allergy Clin. Immunol. Publication Date: 1988 Dec |
Date Detail:
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Created Date: 1989-02-06 Completed Date: 1989-02-06 Revised Date: 2007-11-14 |
Medline Journal Info:
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Nlm Unique ID: 1275002 Medline TA: J Allergy Clin Immunol Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 998-1005 Citation Subset: AIM; IM |
Affiliation:
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Division of Molecular Virology and Immunology, Georgetown University Medical Center, Rockville, Md. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Allergens
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administration & dosage* Anaphylaxis / immunology, pathology Cytoplasmic Granules / immunology, ultrastructure Humans Mast Cells / immunology, ultrastructure* Rhinitis, Allergic, Seasonal / immunology, pathology Skin / ultrastructure* Skin Tests |
| Grant Support | |
ID/Acronym/Agency:
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N01-AI-22665/AI/NIAID NIH HHS; N01-AI-72623/AI/NIAID NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Allergens |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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