Document Detail

Ultrastructural changes in atherosclerotic plaques following the instillation of airborne particulate matter into the lungs of rabbits.
MedLine Citation:
PMID:  20847974     Owner:  NLM     Status:  MEDLINE    
BACKGROUND: Epidemiological studies have established that cardiovascular events account for the greatest number of air pollution-related deaths. However, the underlying structural changes are still unknown.
OBJECTIVE: To investigate changes in the ultrastructure of atherosclerotic plaques in Watanabe heritable hyperlipidemic (WHHL) rabbits following the instillation of ambient particulate matter air pollution (particles smaller than 10 µm in diameter) into the lungs.
METHODS: WHHL rabbits (n=8) exposed to 5 mg of ambient particles (Environmental Health Centre - 1993 [EHC-93]; suspended in saline and instilled in the airway) twice per week for four weeks were compared with control WHHL rabbits (n=8) treated with saline alone.
RESULTS: All abdominal aortic plaques were examined using light and electron microscopy, which showed the following: increased accumulation of macrophage-derived foam cells immediately below the endothelial plaque surface (P=0.04); increased contact between these foam cells and the dense subendothelial extracellular matrix (P<0.005) with reduction (P<0.0001) and fragmentation (P<0.0001) of this matrix; and emigration of macrophage- derived foam cells from the plaques in exposed rabbits. In addition, immunohistochemistry verified the presence of type IV collagen in the thickened extracellular matrix material subtending the endothelium.
CONCLUSIONS: The ultrastructure of atherosclerotic plaques in EHC-93- instilled rabbits differed from the ultrastructure observed in rabbits that did not receive EHC-93. These ultrastructural differences are consistent with greater endothelial instability in the plaques of atherosclerosis-prone rabbits.
Erin M Tranfield; Stephan F van Eeden; Kazuhiro Yatera; James C Hogg; David C Walker
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  The Canadian journal of cardiology     Volume:  26     ISSN:  1916-7075     ISO Abbreviation:  Can J Cardiol     Publication Date:    2010 Aug-Sep
Date Detail:
Created Date:  2010-09-17     Completed Date:  2010-10-15     Revised Date:  2011-08-03    
Medline Journal Info:
Nlm Unique ID:  8510280     Medline TA:  Can J Cardiol     Country:  Canada    
Other Details:
Languages:  eng     Pagination:  e258-69     Citation Subset:  IM    
St Paul's Hospital, University of British Columbia, Vancouver.
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MeSH Terms
Aorta, Abdominal / ultrastructure*
Atherosclerosis / pathology*
Disease Models, Animal
Endothelium, Vascular / ultrastructure
Hyperlipoproteinemia Type II / pathology*
Leukocyte Count
Lung / pathology,  ultrastructure*
Microscopy, Electron
Monocytes / ultrastructure*
Particulate Matter / toxicity*
Reference Values
Grant Support
//Canadian Institutes of Health Research
Reg. No./Substance:
0/Particulate Matter

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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