| Ultrastructural cell wall characteristics of clinical gentamycin-resistant Staphylococcus aureus isolates. | |
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MedLine Citation:
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PMID: 23338781 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
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The frequent use of gentamycin (GM) ointment for the treatment of skin infections has led to an increase in the number of GM-resistant clinical isolates of Staphylococcus aureus. We examined the ultrastructural characteristics of 14 clinical strains of S. aureus by transmission electron microscopy. Seven of these isolates were GM-resistant, and seven isolates were GM-sensitive. We found that the cell wall of GM-resistant strains (32.24 ± 5.99 nm) was significantly thicker than that of GM-sensitive strains (19.02 ± 2.72 nm). We genetically characterized these isolates by polymerase chain reaction, targeting the genes for three aminoglycoside-modifying enzymes, aac(6')-aph(2''), aph(3')-III, and ant(4')-I. All GM-resistant strains tested carried the gene encoding aac(6')-aph(2''). However, we were unable to establish a link between a specific gene and cell wall thickening, because one GM-resistant strain was also positive for aph(3')-III. We also demonstrated that a GM-resistant mutant strain, derived in vitro from a GM-sensitive S. aureus parent strain (209P), also exhibited a thickened cell wall. These results strongly suggest that a thickened cell wall is a common ultrastructural characteristic of GM-resistant S. aureus clinical strains. |
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Authors:
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Kenji Fukutsuji; Sakuo Yamada; Tamotsu Harada |
Publication Detail:
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Type: JOURNAL ARTICLE Date: 2013-1-22 |
Journal Detail:
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Title: Medical molecular morphology Volume: - ISSN: 1860-1499 ISO Abbreviation: Med Mol Morphol Publication Date: 2013 Jan |
Date Detail:
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Created Date: 2013-1-22 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 101239023 Medline TA: Med Mol Morphol Country: - |
Other Details:
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Languages: ENG Pagination: - Citation Subset: - |
Affiliation:
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Department of Otolaryngology, Kawasaki Medical School, 577 Matsusima, Kurasiki, Okayama, Japan, fuku29fukusuke@yahoo.co.jp. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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