| Ultrastructural analysis of cell fusion in yeast. | |
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MedLine Citation:
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PMID: 18979245 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The process of creating a single cell from two progenitor cells requires molecular precision to coordinate the events leading to cytoplasmic continuity while preventing lethal cell lysis. Cell fusion characteristically involves the mobilization of fundamental processes, including signaling, polarization, adhesion, and membrane fusion. The yeast Saccharomyces cerevisiae is an ideal model system for examining the events of this critical and well-conserved process. Researchers employ yeast cells because they are rapidly growing, easy to manipulate, amenable to long-term storage, genetically tractable, readily transformed, and nonhazardous. The genetic and morphological characterizations of cell fusion in wild-type and fusion mutants have helped define the mechanism and temporal regulation required for efficient cell fusion. Ultrastructural studies, in particular, have contributed to the characterization of and revealed striking similarities within cell fusion events in higher organisms. This chapter details two yeast cell fusion ultrastructural methods. The first utilizes an ambient temperature chemical fixation, and the second employs a combination of high-pressure freezing and freeze substitution. |
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Authors:
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Alison E Gammie |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Methods in molecular biology (Clifton, N.J.) Volume: 475 ISSN: 1064-3745 ISO Abbreviation: Methods Mol. Biol. Publication Date: 2008 |
Date Detail:
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Created Date: 2008-11-03 Completed Date: 2008-11-24 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9214969 Medline TA: Methods Mol Biol Country: United States |
Other Details:
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Languages: eng Pagination: 197-211 Citation Subset: IM |
Affiliation:
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Department of Molecular Biology, Princeton University, Princeton, NJ, USA. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Cytological Techniques
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methods* Reproduction Saccharomyces cerevisiae / cytology*, ultrastructure* |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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